Nanozoomer digital pathology view

Manufactured by Hamamatsu Photonics

The NanoZoomer digital pathology view is a high-performance digital slide scanner developed by Hamamatsu Photonics. It is capable of scanning and digitizing glass slides with high image quality and resolution, enabling the conversion of physical pathology samples into digital format for various applications.

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Lab products found in correlation

Nanozoomer xr, by Hamamatsu Photonics (1 mentions) Nanozoomer xr digital slide scanner, by Hamamatsu Photonics (1 mentions) Vectamount permanent mounting medium, by Vector Laboratories (1 mentions) Mayer s haematoxylin, by Vector Laboratories (1 mentions) Immedge hydrophobic barrier pap pen, by Vector Laboratories (1 mentions)

2 protocols using nanozoomer digital pathology view

Quantitative Histology and RNA Analysis

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Hematoxylin and eosin and RNAscope slides were scanned with a NanoZoomer-XR digital slide scanner (Hamamatsu). Images were processed with NanoZoomer digital pathology view (Hamamatsu), and quantification was done with Fiji.

Lopez J.P., Brivio E., Santambrogio A., De Donno C., Kos A., Peters M., Rost N., Czamara D., Brückl T.M., Roeh S., Pöhlmann M.L., Engelhardt C., Ressle A., Stoffel R., Tontsch A., Villamizar J.M., Reincke M., Riester A., Sbiera S., Fassnacht M., Mayberg H.S., Craighead W.E., Dunlop B.W., Nemeroff C.B., Schmidt M.V., Binder E.B., Theis F.J., Beuschlein F., Andoniadou C.L, & Chen A. (2021). Single-cell molecular profiling of all three components of the HPA axis reveals adrenal ABCB1 as a regulator of stress adaptation. Science Advances, 7(5), eabe4497.

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mRNA in situ Hybridization Using RNAscope Kits

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All mRNA in situ hybridisations were performed using the RNAscope singleplex or duplex chromogenic kits (Advanced Cell Diagnostics) on formalin fixed paraffin embedded sections processed as described in the above section. The protocol followed the manufacturer’s instructions with slight modifications. ImmEdge Hydrophobic Barrier PAP Pen (Vector Laboratories, H-4000) was used to draw a barrier around section while air-drying following the first ethanol washes. Pretreatment followed the standard length of time for pituitaries (12 min), while embryos were boiled for 10 min. For singleplex, the protocol proceeded to follow the instructions exactly. For duplex, Amplification nine was extended to 1 hr and the dilution of the Green Detection reagent was increased to 1:30. For both protocols, sections were counterstained with Mayer’s Haematoxylin (Vector Laboratories, H-3404), left to dry at 60°C for 30 min before mounting with VectaMount Permanent Mounting Medium (Vector Laboratories, H-5000). Slides were scanned using a Nanozoomer-XR Digital Slide Scanner (Hamamatsu) and processed using Nanozoomer Digital Pathology View (Hamamatsu).

Russell J.P., Lim X., Santambrogio A., Yianni V., Kemkem Y., Wang B., Fish M., Haston S., Grabek A., Hallang S., Lodge E.J., Patist A.L., Schedl A., Mollard P., Nusse R, & Andoniadou C.L. (2021). Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells. eLife, 10, e59142.

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