Waterborne bacterial strains were isolated from surface water samples. Microorganisms were cultured on a standard nutrient agar medium (Nutrient LAB-AGAR™, Biocorp) for 48 hours at 26 °C. Pure bacteria strains were obtained using the reduction culturing technique and as such, were used for further studies.
Nutrient lab agar
Manufactured by Biocorp
Sourced in Poland
Nutrient LAB-AGAR is a solid growth medium used for cultivating and identifying microorganisms. It provides essential nutrients and a stable agar-based substrate to support the growth of a wide range of bacterial and fungal species.
Automatically generated - may contain errors
Lab products found in correlation
Bacillus cereus, by American Type Culture Collection (1 mentions)
Enterococcus hirae, by American Type Culture Collection (1 mentions)
Legionella pneumophila, by American Type Culture Collection (1 mentions)
Aspergillus brasiliensis, by American Type Culture Collection (1 mentions)
Sabouraud dextrose lab agar, by Biocorp (1 mentions)
Pseudomonas aeruginosa, by American Type Culture Collection (1 mentions)
Staphylococcus aureus, by American Type Culture Collection (1 mentions)
Candida albicans, by American Type Culture Collection (1 mentions)
Alamarblue reagent, by Bio-Rad (1 mentions)
3 protocols using nutrient lab agar
1
Isolation and Cultivation of Waterborne Bacteria
2
Antimicrobial Potential Evaluation
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The biological activity of the samples was studied using a disk diffusion method against both model and waterborne bacteria strains isolated from the natural environment. The bacterial strains were spread in a form of lines on the surface of Petri dishes with solid nutrient agar (Nutrient LAB-AGAR™, Biocorp). Tested samples were placed directly onto the lines with a respective distance between each other. Such prepared plates were then incubated for 48 hours at 26 °C. Petri dishes were then photographed. The photographs were used for measuring the growth inhibition zones around the exanimated samples. The final result of the growth inhibition zone was calculated as an average from ten repetitions, added with standard deviation.
3
Antimicrobial Susceptibility Screening
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The microorganisms used in this study represent pathogenic species commonly associated with healthcare and household environments. Microorganisms were obtained from MediMark Europe and TCS Biosciences Ltd. and consisted of the Gram-positive strains Enterococcus hirae (ATCC 10541) (MediMark), Bacillus cereus (ATCC 10876) (TCS), and Staphylococcus aureus (ATCC 6538) (MediMark); the Gram-negative strains Escherichia coli (ATCC 10536) (MediMark), Pseudomonas aeruginosa (ATCC 15442) (TCS), and Legionella pneumophila (ATCC 33152) (TCS); and the fungi Aspergillus brasiliensis (ATCC 16404) (MediMark), and Candida albicans (ATCC 10231) (MediMark). Bacteria and fungi were maintained in the Department of Chemical Biology and Bioimaging, Wroclaw University of Science and Technology. All bacterial strains were subcultured from the original culture and maintained on Nutrient LAB-AGAR™ (Biocorp Poland) plates at 4 °C and grown at 37 °C when required. All fungal strains were subcultured from the original culture and kept in Sabouraud Dextrose LAB-AGAR™ (Biocorp Poland) plates at 4 °C and grown at 28 °C when required. The Alamarblue® reagent was obtained from Bio-Rad-Antibodies UK. Microplates were obtained from NEST Biotechnology Co., Ltd. China.
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