Phadia immunocap system ecp feia

Serum ECP was measured using the Phadia ImmunoCAP system ECP FEIA (Phadia AB, Uppsala, Sweden), according to the manufacturer's instructions. Briefly, anti-ECP covalently coupled to ImmunoCAP, binds with the ECP in the patient's serum. After washing, enzyme-labeled antibodies against ECP were added to form a complex. After incubation, unbound enzyme-anti-ECP was washed away and the bound complex was then incubated with a developing solution. The reaction was stopped, the fluorescence of the eluate was measured and the response transformed to concentration levels using a calibration curve. The normal range for serum ECP in the patient population that was established in the laboratory was 2.00–15.7 μg/l. Therefore, values above this were considered elevated for the study population. The maximum value was similar to that of a previous study (13.3 μg/l) (37 (link)). To ensure quality control the laboratory participated in an external quality assessment program (Phadia: Quality Club ECP [52-5213-99/05]).