Anti phosphojak2

Dulbecco’s Modified Eagle’s Medium (DMEM), Fetal Bovine Serum (FBS), L-glutamine, penicillin/streptomycin, phosphate buffered saline (PBS), bovine serum albumin (BSA), protease inhibitors, trypan blue, lipopolysaccharide from E. coli (LPS), trichloroacetic acid (TCA), sulphorhodamine B (SRB), Tris base (Tris[hydroxymethyl]aminomethane), pinosylvin (HPLC, ≥97%), and resveratrol were purchased from Sigma-Aldrich S.p.a. (Milan, Italy). ECL System was from Bio-Rad. RAW 264.7 cells were purchased from ATCC, Glasgow, UK (No. TIB-71), employed Abs anti-phosphoJak2 (#PA538287), anti-Jak2 (#PA511267), anti-phosphoStat3 (#PA5121259), and anti-Stat3(#PA5120138) were from ThermoFisher Scientific. Anti-β-actin (AC-15; sc-69879) was from Santa Cruz Biotechnology, Inc., Heidelberg, Germany. Water and acetonitrile were HPLC-grade, while all other solvents were reagent-grade, obtained by VWR International s.r.l. (Milan, Italy).

Folin-Ciocalteu reagent, aluminum chloride, chlorogenic acid, quercetin, bergapten, fetal bovine serum (FBS), L-glutamine, penicillin/streptomycin, Dulbecco’s Modified Eagle’s Medium (DMEM), bovine serum albumin (BSA), protease inhibitors, trypan blue, phosphate buffered saline (PBS), LPS, trichloroacetic acid (TCA), sulphorhodamine B (SRB) were purchased by Sigma-Aldrich S.p.a. (Milan, Italy). ECL System was from Bio-Rad. RAW 264.7 cells were purchased by ATCC, UK (No. TIB-71). The Abs employed were anti-phosphoJak2 (#PA538287), anti-Jak2 (#PA511267), anti-phosphoStat3 (#PA5121259), anti-Stat3(#PA5120138), (all from ThermoFisher Scientific, Waltham, MA, USA), anti-β-actin (AC-15; sc-69879) from Santa Cruz Biotechnology, Inc., Heidelberg, Germany. All other reagents were obtained by VWR International s.r.l. (Milan, Italy).