Mrc1 pe

Manufactured by BioLegend

The MRC1-PE is a fluorescently labeled antibody targeting the Mannose Receptor CD206. It is designed for use in flow cytometry applications to detect and quantify cells expressing the MRC1 (CD206) surface marker.

Automatically generated - may contain errors

Lab products found in correlation

Cd14 pe cy7, by BioLegend (2 mentions) Cd40 apc, by BioLegend (2 mentions) Mhcii fitc, by BioLegend (2 mentions) Cd80 pe, by BioLegend (2 mentions) Cd11a pe, by BioLegend (2 mentions) Cd86 apc cy7, by BioLegend (2 mentions) Pd l1 bv421, by BioLegend (2 mentions) Tlr2 apc, by BioLegend (2 mentions) Siglec1 fitc, by BioLegend (2 mentions) Lsr 2, by BD (1 mentions) Cd69 pe, by BioLegend (1 mentions)

2 protocols using mrc1 pe

Immunophenotyping of Immune Cells

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For all experiments, cells were lifted by gentle scrapping, washed with PBS, and stained with MHCII-FITC (BioLegend, Cat no. 107606), CD40-APC (BioLegend, Cat no. 124612), PDL1-BV421 (BioLegend, Cat no. 124315), CD80-PE (BioLegend, Cat no. 104708), and CD86-APC-Cy7 (BioLegend, Cat no. 104708) TLR2-APC (Biolegend, Cat no. 153006) MRC1-PE (Biolegend, Cat no. 141706) Siglec1-FITC (Biolegend, Cat no. 142406) CD14-PE-Cy7 (Biolegend, Cat no. 123316) CD11a-PE (Biolegend, Cat no. 153103) (all diluted 1:400 in PBS). Cells were then washed 3 times in PBS and fixed with 1% formaldehyde (J.T. Baker, Cat no. JTB-2106-01) in PBS. Flow cytometry was performed on a BD LSR II or an Attune CytPix at the MSU Flow Cytometry Core, and data were analyzed using FlowJo (Version 10.8.1).

Ankley L.M., Conner K.N., Vielma T.E., Thapa M, & Olive A.J. (2023). GSK3α/β restrains IFNγ-inducible costimulatory molecule expression in alveolar macrophages, limiting CD4+ T cell activation. bioRxiv.

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Multiparametric Flow Cytometry Analysis

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For all experiments, the cells were lifted by gentle scraping, washed with PBS, and stained with MHC-II-FITC (BioLegend, catalog no. 107606), CD40-APC (BioLegend, catalog no. 124612), PDL1-BV421 (BioLegend, catalog no. 124315), CD80-PE (BioLegend, catalog no. 104708), CD86-APC-Cy7 (BioLegend, catalog no. 104708), TLR2-APC (BioLegend, catalog no. 153006), MRC1-PE (BioLegend, catalog no. 141706), Siglec1-FITC (BioLegend, catalog no. 142406), CD14-PE-Cy7 (BioLegend, catalog no. 123316), CD11a-PE (BioLegend, catalog no. 153103), and CD69-PE (BioLegend, catalog no. 104508) (all diluted 1:400 in PBS). The cells were then washed three times in PBS and fixed with 1% formaldehyde (J. T. Baker, catalog no. JTB-2106-01) in PBS. Flow cytometry was performed on a BD LSR II or an Attune CytPix at the Michigan State University Flow Cytometry Core, and the data were analyzed using FlowJo (version 10.8.1).

Ankley L.M., Conner K.N., Vielma T.E., Godfrey J.J., Thapa M, & Olive A.J. (2024). GSK3α/β Restrain IFN-γ–Inducible Costimulatory Molecule Expression in Alveolar Macrophages, Limiting CD4+ T Cell Activation. ImmunoHorizons, 8(2), 147-162.

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