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The H-105 is a laboratory centrifuge designed for general-purpose applications. It features a compact and durable construction, accommodating a variety of sample volumes and rotor configurations. The centrifuge is capable of achieving high speeds and g-forces, making it suitable for a range of common laboratory procedures.

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Lab products found in correlation

H 300, by Santa Cruz Biotechnology (2 mentions) Sc 126, by Santa Cruz Biotechnology (1 mentions) Fl 393, by Santa Cruz Biotechnology (1 mentions) H 150, by Santa Cruz Biotechnology (1 mentions) H 123, by Santa Cruz Biotechnology (1 mentions) Topo 2 h 8, by Santa Cruz Biotechnology (1 mentions) Nuclear and cytoplasmic protein extraction kit, by Beyotime (1 mentions) Goat anti rabbit igg, by Beyotime (1 mentions) Quantity one software, by Bio-Rad (1 mentions) Ecl western blotting detection reagent, by Beyotime (1 mentions)

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Anti-HO-1 (H-105; (2 citations)

4 protocols using h 105

1

Immunoprecipitation and Western Blot Analysis

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Total protein (100 μg) from indicated NEs was e-IPed with antibodies against Pin1 polyclonal (H-123, Santa Cruz Biotechnology), tau polyclonal (H-150, Santa Cruz Biotechnology), PARN polyclonal (H-105, Santa Cruz Biotechnology), p53 monoclonal (SC-126, Santa Cruz Biotechnology), and p53 polyclonal (FL-393, Santa Cruz Biotechnology) as described (Cevher et al., 2010 (link); Nazeer et al., 2011 (link)). For tau detection in Western blot analysis, tau-13 and tau-1, which is reactive when Ser198/199/202 are not phosphorylated, mouse monoclonal antibodies were used (Alonso et al., 2010 (link)). Other antibodies in Western blot analysis: Topo II (H-8, Santa Cruz Biotechnology), PARN (A303-562A, Bethyl), Actin (A2066, Sigma), Pin1 (A302-316A, Bethyl), α-tubulin (2871-1, Epitomics), EGFR (1005, Santa Cruz Biotechnology), and H2B (8135, Cell Signaling). Because antibody tau-1 recognizes tau only when it is not phosphorylated, the blots were pretreated with alkaline phosphatase (196 units/ml) in 0.1 M Tris, pH 8.0, and 1 mM phenylmethylsulfonyl fluoride for 5 h prior to incubation with the primary antibody (Alonso et al., 2001 (link)).
Baquero J., Varriano S., Ordonez M., Kuczaj P., Murphy M.R., Aruggoda G., Lundine D., Morozova V., Makki A.E., Alonso A.D, & Kleiman F.E. (2019). Nuclear Tau, p53 and Pin1 Regulate PARN-Mediated Deadenylation and Gene Expression. Frontiers in Molecular Neuroscience, 12, 242.
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2

Immunoprecipitation of PARN and Ago-2

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100 μg total protein from the indicated NEs were immunoprecipitated (IPed) with polyclonal antibody against either PARN (H-105, Santa Cruz Biotechnology) or Ago-2 as described (4 (link),20 (link)).
Zhang X., Devany E., Murphy M.R., Glazman G., Persaud M, & Kleiman F.E. (2015). PARN deadenylase is involved in miRNA-dependent degradation of TP53 mRNA in mammalian cells. Nucleic Acids Research, 43(22), 10925-10938.
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3

PARN and Ago-2 Colocalization in HCT116 Cells

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HCT116 cells were seeded onto glass coverslips at 5 × 104 cells/ml, immunostained for PARN (H-105, Santa Cruz Biotechnology) and Ago-2 (H-300, Santa Cruz) as described (26 (link)). Cells were examined under a Nikon TIRF SIM laser spinning disk fluorescence confocal microscope.
Zhang X., Devany E., Murphy M.R., Glazman G., Persaud M, & Kleiman F.E. (2015). PARN deadenylase is involved in miRNA-dependent degradation of TP53 mRNA in mammalian cells. Nucleic Acids Research, 43(22), 10925-10938.
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4

Nuclear Protein Extraction and Western Blot Analysis

Cited 1 time
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The right lungs were removed and nuclear fractions were prepared using a Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime Institute of Biotechnology, China) according to the manufacturer's protocol. Western blot analysis was carried out as described [23 (link)]. Primary antibodies (working concentration) used were rabbit polyclonal antibodies against mice Nrf2 (1 : 2000, H-300, Santa Cruz Biotechnology, CA), HO-1 (1 : 1000, H-105, Santa Cruz Biotechnology, CA), and Lamin B1 (1 : 2000, H-90, Santa Cruz Biotechnology, CA). The HRP-conjugated secondary antibody was goat anti-rabbit IgG (Beyotime Institute of Biotechnology, China) used at 1 : 2000. Lamin B was used as an internal control. The ECL Western blotting detection reagents (Beyotime Institute of Biotechnology, China) were used for visualization of the protein bands. The intensities of the bands were analyzed with quantity one software (Bio-Rad, Hercules, CA).
Jiang Y., Zhou Z., Meng Q.T., Sun Q., Su W., Lei S., Xia Z, & Xia Z.Y. (2015). Ginsenoside Rb1 Treatment Attenuates Pulmonary Inflammatory Cytokine Release and Tissue Injury following Intestinal Ischemia Reperfusion Injury in Mice. Oxidative Medicine and Cellular Longevity, 2015, 843721.
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