After treatment, samples were opened aseptically, and the contents were transferred to a sterile stomacher bag (Interscience, St. Nom La Breteche, France). A 10−1 dilution of the sample was prepared in maximum recovery diluent (Oxoid, CM733, Basingstoke, United Kingdom). The dilution was homogenized for 60 s at high speed in a Seward stomacher (Lab blender 400, Bury St. Edmunds, United Kingdom). Further 10-fold dilutions were prepared in 9 mL Maximum Recovery Diluent (MRD). Total viable counts (TVC) were enumerated by spread-plating onto tryptone soya agar with 0.6% yeast extract plates (TSAYE; Oxoid, Basingstoke, United Kingdom), with aerobic incubation at 30 °C for 48 h. Lactic acid bacteria (LAB) were enumerated on de Man Rogosa and Sharp agar (Oxoid, Basingstoke, United Kingdom) by pour-plating with overlay and incubating aerobically at 30 °C for 72 h. Enterobacteriaceae were enumerated using Violet Red Bile Glucose Agar (Oxoid, Basingstoke, United Kingdom) by pour-plating with overlay and incubating aerobically at 37 °C for 72 h. Yeasts and moulds (YM) were enumerated on Dextrose Rose-Bengal Chloramphenicol agar (Oxoid, Basingstoke, United Kingdom) with incubation at 25 °C for 72 and 120 h. Each sample was plated in duplicate, and the results were expressed as log colony-forming units (CFU)/g.
Cm733
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
The CM733 is a laboratory centrifuge designed for general-purpose applications. It features a compact and durable construction, as well as a microprocessor-controlled motor to ensure reliable and consistent performance.
Automatically generated - may contain errors
Lab products found in correlation
Sterile stomacher bag, by Interscience (1 mentions)
Violet red bile glucose agar, by Thermo Fisher Scientific (1 mentions)
400 lab blender, by Seward Medical (1 mentions)
Plate count agar pca, by Thermo Fisher Scientific (1 mentions)
Rose bengal chloramphenicol agar, by Thermo Fisher Scientific (1 mentions)
Violet red bile glucose agar vrbga, by Thermo Fisher Scientific (1 mentions)
Anaerocult a, by Merck Group (1 mentions)
3 protocols using cm733
1
Enumeration of Microbial Populations in Food Samples
2
Microbial Enumeration in Fruit Juices
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At each sampling interval, juice samples were opened aseptically and a suitable dilution series was prepared in maximum recovery diluent (MRD) (Oxoid code CM733, Oxoid, Basingstoke, UK) and the appropriate dilutions were prepared. Total viable counts (TVC) were enumerated by spread plating onto plate count agar (PCA) (Oxoid, Basingstoke, UK), after aerobic incubation at 30 °C for 48 h. Lactic acid bacteria were enumerated on de Man Rogosa and Sharp agar (MRS) (Oxoid, Basingstoke, UK) by pour plating and incubating at 30°C for 72 hours. Enterobacteriaceae were enumerated onto Violet Red Bile Glucose Agar (VRBGA) (Oxoid, Basingstoke, UK) by pour plating and incubating at 37 °C for 72 hours.
Yeasts and moulds were enumerated on Rose-Bengal Chloramphenicol agar (Oxoid, Basingstoke, UK) with incubation at 25ºC for 72 and 120 hours. Each sample was plated in duplicate and the results (the mean of the two plates) were expressed as log10CFU/ml of juice.
Yeasts and moulds were enumerated on Rose-Bengal Chloramphenicol agar (Oxoid, Basingstoke, UK) with incubation at 25ºC for 72 and 120 hours. Each sample was plated in duplicate and the results (the mean of the two plates) were expressed as log10CFU/ml of juice.
3
Microbiological Analysis of Cold-Smoked Fish
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From each pack, 30 g of cold-smoked fish was taken aseptically (10 g + 10 g + 10 g, from 3 different parts of the sample) and homogenized for 90 s in a stomacher (Seward 400). Ten grams of the mixture was aseptically taken and decimally diluted in sterile Maximum Recovery Diluent (CM 733; Oxoid) and homogenized for 20 s. Aerobic plate counts were performed on spread plates of Long and Hammer's medium (LH) (Van Spreekens, 1974) with additional 1% w/v NaCl, incubated at 15 • C for 5-7 d. Counts of lactic acid bacteria (LAB) were obtained from pour plates of NAP medium, pH 6.7 (Davidson & Cronin, 1973) , incubated anaerobically (Anaerocult A, Merck) at 21 • Cfor 5d. Enterobacteriaceae counts were obtained from pour plates of 5 ml tryptone soya agar (TSA), and after 2-h resuscitation of damaged cells at 20-25 • C plates were overlaid with 12-15 ml of violet red bile glucose agar (VRBGA). Typical Enterobacteriaceae colonies were counted after 2 d of incubation at 30 • C. To assess the selectivity of the different media, representative colonies were selected from plates and the following tests were performed: cell morphology, gram stain, and catalase and oxidase tests.
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