For GFP-imaging, A. thaliana plants, in which the promoter of the PCO1 gene was fused to a GUS-GFP reporter, were grown and inoculated with A. tumefaciens as described above. Crown galls were analyzed using a Leica M205 FA stereomicroscope equipped with a Leica DFC450 C camera at three different developmental stages (7, 14, and 28 dpi). GFP was detected using a band pass ET GFP Filter set (Leica).
Et gfp filter set
Manufactured by Leica
The ET GFP Filter set is a high-performance optical filter designed for the detection and visualization of green fluorescent protein (GFP) in fluorescence microscopy. The filter set consists of an excitation filter, a dichroic mirror, and an emission filter, all optimized for the specific excitation and emission spectra of GFP. The set provides efficient separation of the excitation and emission light, enabling clear and precise imaging of GFP-labeled samples.
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Lab products found in correlation
2 protocols using et gfp filter set
1
GFP Imaging of A. thaliana Crown Galls
2
Visualizing LDH Transgenes in Larvae and Pupae
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Expression of the LDH-GFPGenomicand LDH-mCherryGenomic transgenes in intact larvae and pupae was visualized without fixation using a MZ10F microscope with a EL6000 light source. GFP was visualized with the Leica ET GFP filter set (470 nm excitation filter; 525 emission filter). mCherry was visualized using the Leica ET mCherry filter set (560 nm excitation filter; 630 nm emission filter). Images were taken using a Leica MC170 HD microscope camera.
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