Poly l lysine hbr pll

Manufactured by Merck Group

Poly(l-lysine) HBr (PLL) is a water-soluble, cationic polymer commonly used as a surface coating material in various laboratory applications. Its core function is to provide a positively charged substrate that can facilitate the attachment and immobilization of cells, proteins, and other biomolecules.

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Lab products found in correlation

Dextran sulfate sodium salt, by Merck Group (1 mentions) P akt, by Merck Group (1 mentions) P erk1 2, by Merck Group (1 mentions) Nbd dmpe, by Avanti Polar Lipids (1 mentions)

Close spelling variants of this product

Poly(L-lysine hydrobromide) (PLL-HBr, Poly-L-lysine Poly-Lysine (PLL), L-lysine

2 protocols using poly l lysine hbr pll

Protein Signaling Pathway Analysis

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Poly(l-lysine) HBr (PLL, 15-30 kDa; Sigma-Aldrich), hyaluronan (HA, 200 kDa; Lifecore), and dextran sulfate sodium salt (DXS, 20 kDa; Sigma-Aldrich) were used as-received without further modification. Antibodies included total Akt (CST 4691), cleaved caspase-3 (CST 9661), total Erk (CST 4370), pAkt (CST 4060; S473), pAkt-Alexa Fluor 488 (Millipore CS203310; S473), pErk1/2 (CST 4370; T202/204, T185/187), and pErk1/2-PE (Millipore CS203329; T202/204, T185/187).

Dreaden E.C., Kong Y.W., Morton S.W., Correa S., Choi K.Y., Shopsowitz K.E., Renggli K., Drapkin R., Yaffe M.B, & Hammond P.T. (2015). Tumor-Targeted Synergistic Blockade of MAPK and PI3K from a Layer-by-Layer Nanoparticle. Clinical cancer research : an official journal of the American Association for Cancer Research, 21(19), 4410-4419.

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Polycation-Lipid Nanomaterial Synthesis

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Sodium hyaluronate (HA, Lifecore Biomedical, 360 kDa), poly-L-lysine HBr (PLL, Sigma), PLL of 15-30 kDa (PLL28, where the average M w is 28 kDa) and PLL of 150-300 kDa (PLL280) were used. Poly(ethylenimine) (PEI, Sigma), 2-oleoyl-1-palmitoylsn-glycero-3-phosphocholine (POPC, Avanti Polar Lipids, M w : 785.6), 2-oleoyl-1-palmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt (POPG, Avanti Polar Lipids, M w : 771.0), N-(NBD-aminododecanoyl)-1,2-ditetradecanoyl-sn-glycero-3-phosphoethanolamine sodium salt (NBD-DMPE, Avanti Polar Lipids, M w: 816.0), cholesterol (Chol, Sigma, M w : 386.7), lysozyme (chicken egg white, CalBioChem, M w : 14.3 kDa), and α-lactalbumin (bovine milk, type II, calcium depleted, Sigma, M w : 14.2 kDa) were also used. 10 mM Tris buffer solution containing 15 mM NaCl at pH 7.4 and 0.1 M carbonate buffer solution at pH 9.0 were prepared using distilled water and chemicals purchased from Sigma. Other chemicals and reagents were of analytical grade.
Chemical structures of the lipids and polymers used in this study are provided in Fig. S1. †

Vikulina A., Wulf A., Guday G., Fakhrullin R, & Volodkin D. (2023). A lipid membrane supported on an artificial extracellular matrix made of polyelectrolyte multilayers: towards nanoarchitectonics at the cellular interface. Nanoscale, 15(5).

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