Log In Sign up
The largest database of trusted experimental protocols

Anti dlst

Manufactured by Abnova
Visit Supplier
Sourced in United States

Anti-DLST is a laboratory reagent used for the detection and quantification of the DLST (Dihydrolipoamide S-succinyltransferase) protein in biological samples. It is a component of the pyruvate dehydrogenase complex and plays a role in energy metabolism.

Automatically generated - may contain errors

Lab products found in correlation

G box chemi xt4, by Syngene (3 mentions) Phosphatase inhibitor cocktail, by Boston BioProducts (2 mentions) Supersignal west pico chemiluminescence, by Thermo Fisher Scientific (2 mentions) Anti actin, by Santa Cruz Biotechnology (2 mentions) Anti rabbit antibodies, by Thermo Fisher Scientific (1 mentions) Anti ldha, by Cell Signaling Technology (1 mentions) Anti actin, by Merck Group (1 mentions) Anti icn1, by Cell Signaling Technology (1 mentions) Anti myc, by Santa Cruz Biotechnology (1 mentions) Anti pten, by Cell Signaling Technology (1 mentions) Halt proteinase and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) Las 3000 imaging system, by Fujifilm (1 mentions)

3 protocols using anti dlst

1

Protein Expression Analysis in Cell Lysates

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in RIPA buffer supplemented with 1x Halt proteinase inhibitor (87786, Thermo Scientific) and phosphatase inhibitor cocktail (BP479, Boston Bioproducts, Milford, MA, USA). The primary antibodies used include anti-DLST (H00001743, Abnova, Taipei, Taiwan), anti-IDH1 (LS-C497935, Life span bioscience, Seattle, WA, USA), anti-IDH2 (LS-C373619, Life span bioscience), anti-LDHA (#2012, Cell Signaling Technology, Danvers, MA, USA), and anti-ACTIN (sc-47778, Santa Cruz Biotechnology, Dallas, TX, USA). Secondary antibodies include anti-mouse (31430, Thermo Scientific) or anti-rabbit antibodies (65-6120, Thermo Scientific). Chemiluminescence Supersignal West Pico was from Thermo Scientific (Cat# 34080). Autoradiographs were imaged using a G: BOX Chemi XT4 (Syngene, Bengaluru, India).
Wang Y., Shen N., Spurlin G., Korm S., Huang S., Anderson N.M., Huiting L.N., Liu H, & Feng H. (2022). α-Ketoglutarate-Mediated DNA Demethylation Sustains T-Acute Lymphoblastic Leukemia upon TCA Cycle Targeting. Cancers, 14(12), 2983.
+ Open protocol
+ Expand
2

Western Blot Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in RIPA buffer supplemented with 1× Halt proteinase inhibitor (87786, Thermo Scientific) and phosphatase inhibitor cocktail (BP479, Boston Bioproducts). Primary antibodies include anti-DLST (H00001743, Abnova) and anti-ACTIN (sc-47778, Santa Cruz Biotechnology). Secondary antibodies included goat anti-mouse (31430, Thermo Scientific) or anti-rabbit HRP antibodies (65-6120, Thermo Scientific). Chemiluminescence Supersignal West Pico was from Thermo Scientific (Cat# 34080). Autoradiographs were imaged using a G: BOX Chemi XT4 (Syngene).
Shen N., Korm S., Karantanos T., Li D., Zhang X., Ritou E., Xu H., Lam A., English J., Zong W.X., Liu C.T., Shirihai O, & Feng H. (2021). DLST-dependence dictates metabolic heterogeneity in TCA-cycle usage among triple-negative breast cancer. Communications Biology, 4, 1289.
+ Open protocol
+ Expand
3

Western Blot Analysis of T-ALL Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Zebrafish and human T-ALL cells were lysed in RIPA buffer supplemented with Halt proteinase and phosphatase inhibitor cocktail (Thermo Scientific, Cambridge, MA, USA). The primary antibodies included anti-MYC (Santa Cruz, Dallas, TX, USA; or Cell Signaling), anti-ICN1 (Cell Signaling, Val1744), anti-PTEN (Cell Signaling), anti-DLST (Abnova, Walnut, CA, USA), anti-SHMT2 (Aviva, London, UK) and anti-ACTIN (Sigma). Secondary antibodies included horseradish peroxidase-conjugated anti-mouse or anti-rabbit antibodies (Pierce, Cambridge, MA, USA). Autoradiographs were imaged with a G:BOX chemi XT4 (Syngene, Frederick, MD, USA) or a LAS-3000 imaging system (Fuji, Valhalla, NY, USA) and a CCD camera and then subjected to quantification analysis with Syngene GeneTools or Fuji software.
Anderson N., Li D., Peng H., Laroche F., Mansour M., Gjini E., Aioub M., Helman D., Roderick J., Cheng T., Harrold I., Samaha Y., Meng L., Amsterdam A., Neuberg D., Denton T., Sanda T., Kelliher M., Singh A., Look A, & Feng H. (2016). The TCA cycle transferase DLST is important for MYC-mediated leukemogenesis. Leukemia, 30(6), 1365-1374.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!