500,000 living cells were pelleted by centrifugation at 400×g for 7 min followed by resuspending the cells in 500 μL Ringer’s Lactate solution (Fresenius-Kabi, Germany) containing 0.5 μM Syto24 nuclear dye (Thermo-Fisher, USA). Cells were then incubated for 90 min at 4 °C followed by addition of 10 mL Ringer’s Lactate solution. Next, cells were centrifuged, supernatant discarded, and cell pellet resuspended in 10 mL growth medium. After another centrifugation, cell pellet was resuspended in growth medium to achieve 125,000 cells per mL.
Ringer s lactate solution
Manufactured by Fresenius
Sourced in Austria
Ringer's lactate solution is a sterile, isotonic intravenous fluid used for fluid and electrolyte replacement. It contains a balanced mixture of sodium, potassium, calcium, and lactate ions, which help maintain the body's fluid and electrolyte balance. The solution is primarily used in medical settings to treat dehydration and electrolyte imbalances.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using ringer s lactate solution
1
Cell Labeling and Quantification
2
Equine Embryo Recovery and Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Embryos were recovered on days seven and eight after ovulation (day 0, ovulation detection) using an intrauterine silicone two-way Foley catheter CH 28 for mares (Minitube). The uterus was flushed four times with 1 L of Ringer’s lactate solution (Fresenius Kabi, Graz, Austria) prewarmed at 38 °C. The fluid recovered from the uterus was filtered through an embryo filter system (75 μm, EmCon embryo filter; Immunosystems, Spring Valley, WI, USA). The solution remaining in the filter cup was placed in a petri dish and analyzed under a stereomicroscope at 40 × magnification. Embryos were washed 10 times in holding medium (Minitube) to remove cellular debris. Embryos were measured before and immediately after the storage period under stereo microscope with an eyepiece micrometer, and morphologically classified according to their stage of development and quality on a scale from 1 (excellent) to 4 (degenerated) as described [32 (link)].
3
Biodistribution of Stromal Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Animal experiments were performed in accordance with the guidelines of the “Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes” and were approved by the national animal care authorities (authorization number: BMWFW-66.019/0024-WF/V/36/2016). Stromal cells from three independent BM and UC donors were expanded for one passage and pooled to limit donor variation. TF-deficient BMSC were sorted to purity as described above immediately before tail vein injection into Fischer rats (1.5 million BMSC per 150 µL Ringer's lactate solution (Fresenius-Kabi, Austria) per injection; n = 3). For comparison, equal numbers of UC stromal cells previously checked for homogenous high TF expression were injected in three additional animals. Rats were sacrificed exactly one hour after stromal cell injection by CO2 and lung, liver and spleen were surgically removed without perfusion before perfusion in 4% paraformaldehyde. Fixed organs were sectioned (4 µm) and processed for hematoxylin/eosin (Sigma-Aldrich, USA /Merck Milipore, Germany) and Masson's trichrome (MORPHISTO, Germany) histochemistry following standard protocols. Pictures were captured using the Olympus slidescanner VS120 at 40x magnification and processed using the Olympus VS-ASW-L100 software.
4
Equine Embryo Recovery and Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Embryos were recovered on days 7 and 8 after ovulation (day 0, ovulation detection) using an intrauterine silicone 2-way Foley catheter CH 28 for mares (Minitube). The uterus was washed four times with 1 L of Ringer's lactate solution (Fresenius Kabi, Graz, Austria) prewarmed at 38 °C. The fluid recovered from the uterus was filtered through an embryo filter system (75 µm, EmCon embryo filter; Immunosystems, Spring Valley, WI, USA). The solution remaining in the filter cup was placed in a petri dish and analyzed under a stereomicroscope at 40 × magnification. Embryos were washed 10 times in holding medium (Minitube) to remove cellular debris. Embryos were measured with a microscopic scale and morphologically classified according to their quality on a scale from 1 (excellent) to 4 (degenerated) as described [28] .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!