Phospho rip1

Manufactured by Cell Signaling Technology

Sourced in United States

Phospho-RIP1 is a laboratory antibody product from Cell Signaling Technology that recognizes the phosphorylated form of the RIP1 (receptor-interacting protein 1) protein. RIP1 is a key regulator of cell signaling pathways involved in inflammation and cell death. The Phospho-RIP1 antibody can be used to detect the phosphorylated state of RIP1 in various experimental systems.

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Lab products found in correlation

Spermidine, by Merck Group (1 mentions) Adalimumab, by Selleck Chemicals (1 mentions) Mmp13, by Abcam (1 mentions) Ha tag, by Santa Cruz Biotechnology (1 mentions) Tnf α, by Abcam (1 mentions) Phospho iκb, by Santa Cruz Biotechnology (1 mentions) Phospho nf κb p65, by Cell Signaling Technology (1 mentions) Anti mouse igg h l alexa fluor 488, by Cell Signaling Technology (1 mentions) Adamts4, by Abcam (1 mentions) Aggrecan, by Merck Group (1 mentions) Adamts5, by Abcam (1 mentions) Nf κb p65, by Cell Signaling Technology (1 mentions) Phospho pras40, by Cell Signaling Technology (1 mentions) Vinculin, by Merck Group (1 mentions) Phospho rb, by Cell Signaling Technology (1 mentions) C parp, by Cell Signaling Technology (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Total erk1 2, by Cell Signaling Technology (1 mentions) Phospho p90rsk, by Cell Signaling Technology (1 mentions) Gapdh 8884, by Cell Signaling Technology (1 mentions)

2 protocols using phospho rip1

Investigating Inflammatory Mediator Modulation

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TNF-α (Catalog #: ab6671), Collagen 10 (Catalog #: ab49945), MMP3 (Catalog #: ab52915), MMP13 (Catalog #: ab39012), iNOS (Catalog #: ab15323), UBA1 (Catalog #: ab34711) Adamts4 (Catalog #: ab1855722) and Adamts5 (Catalog #: ab41037) were purchased from Abcam (MA, USA), Adalimumab (Catalog #: A2010) and Glucosamine (Catalog #: S6400) were purchased from Selleckchem (TX, USA), UBE2N (Catalog #: 6999), phospho-RIP1 (Catalog #: 65746), NF-κB phospho-p65 (Catalog #: 3033), NF-κB p65 (Catalog #: 8242), RIP1 (Catalog #: 3943), Anti-rabbit IgG (H + L) Alexa Fluor 555 (Catalog #: 4413) and Anti-mouse IgG (H + L) Alexa Fluor 488 (Catalog #: 4408) were purchased from Cell Signaling Technology (MA, USA), Vimentin (Catalog #: SC6260), HA-Tag (Catalog #: SC7392), IκB (Catalog #: SC1643) and phospho-IκB (Catalog #: SC8404) were purchased from Santa Cruz Biotechnology (CA, USA), Spermidine (Catalog #: 05292) and Aggrecan (Catalog #: AB1031) were purchased from Sigma (MO, USA), CLYD (Catalog #: 1110-1-AP) and TRIM21 (Catalog #: 12108-1-AP) were purchased from PeproTech (NJ, USA).

Chen Z., Lin C.X., Song B., Li C.C., Qiu J.X., Li S.X., Lin S.P., Luo W.Q., Fu Y., Fang G.B., Wei-Ping L., Saw P.E, & Ding Y. (2020). Spermidine activates RIP1 deubiquitination to inhibit TNF-α-induced NF-κB/p65 signaling pathway in osteoarthritis. Cell Death & Disease, 11(7), 503.

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Western Blot Antibody Panel

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The Western blot method is described in detail in the Supplemental Material. The following antibodies were from Cell Signaling: GAPDH (8884), phospho-ERK1/2 (T202/Y204; 4370P), total ERK1/2 (4695P), phospho-AKT (S473; 4060P), total AKT (2920S), phospho-p90RSK (Ser380; 9341S), c-Myc (5605S), cyclin D1 (2978S), phospho-PRAS40 (Thr246; 2997S), KLF4 (4038S), phospho-Rb (Ser780; 3590S), total Rb (9309S), E2F1 (3742S), c-PARP (9541S), cleaved caspase substrate motif (8698S), phospho-RIP1 (Ser166; 65746S), total RIP1 (3493T), and LC3B (3868T). Other antibodies used were cyclin A (Santa Cruz Biotechonology, sc-751), vinculin (Sigma, V9131), and RRAS2 (Abcam, ab182264).

Liao S., Maertens O., Cichowski K, & Elledge S.J. (2018). Genetic modifiers of the BRD4-NUT dependency of NUT midline carcinoma uncovers a synergism between BETis and CDK4/6is. Genes & Development, 32(17-18), 1188-1200.

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