Transmission electron microscopy (TEM) images were taken on a JEOL 2100 Cryo transmission electron microscope with an accelerating voltage of 200 kV. Fluorescence spectra were recorded on a FluoroMax-P fluorimeter (HORIBA Jobin Yvon Inc., Edison, NJ) equipped with a commercial CW IR laser (980 nm). UV/vis spectra were recorded on a Hewlett–Packard 8453 spectrometer.
Fluoromax p fluorimeter
Manufactured by Horiba
Sourced in United States
The FluoroMax-P fluorimeter is a high-performance spectrofluorometer designed for laboratory use. It is capable of measuring the fluorescence emission and excitation spectra of samples. The FluoroMax-P provides accurate and reliable fluorescence data for a variety of research and analytical applications.
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5 protocols using fluoromax p fluorimeter
1
Characterization of Nanoparticles Using TEM
2
Characterization of Nanoparticles using TEM and Spectroscopy
3
Nanomaterial Characterization Techniques
4
Characterization of Quantum Dot Solutions
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A Hewlett-Packard 8453 UV-visible spectrophotometer was used to determine the electronic absorption properties of the QD solutions. A Jobin Yvon Fluoromax-P fluorimeter was used to study the luminescent properties of the QD solutions. Samples for analysis were prepared by placing approximately 5-10 drops (< 1 mL) of the QD solution into a 1-cm quartz cuvette (Fisher) and filling the cuvette with RO water. Typically, an excitation wavelength of 350 nm was applied for fluorescence measurements. The spectral properties of all QD samples were analyzed at ambient temperatures. The determination of fluorescent quantum yield was performed using a standard method [15 (link), 16 (link)]. Rhodamine 6G (in H2O, Φ = 0.95) was used as the standard for the analysis. The absorbance value of a QD sample at 348 nm was adjusted from 0.10 to 0.01 absorbance units. The integrated fluorescence area was measured from 400 to 800 nm for each of the dilutions. Quantum yield values for QD solutions ranged from 4 to 8%, which is lower than observed with other water-soluble QD systems [17 (link)–19 (link)].
5
Fluorometric Assay for NO-hTPI Quantification
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Levels of NO-hTPI were assayed with a fluorometric technique [28] . Briefly, after S-nitrosylation NO-hTPI was separated from the excess NO-donors using PD SpinTrap G-25 columns. Aliquots corresponding to 50 μg of purified NO-hTPI were dissolved in 190 μL of 60 mM HCl containing 3.2 μM 2,3-diaminonaphthalene with and without 0.2 mM HgCl 2 , and incubated at room temperature. After 10 min, 10 μL of 2.8 N NaOH was added to stabilize the fluorescent 2,3-naphthotriazole product. Fluorescence was measured using a FluoroMax-P fluorimeter (Horiba Jobin Yvon, Edison, NJ, USA) with excitation and emission wavelengths of 363 nm and 450 nm, respectively. Emission intensity was converted to NO-TPI concentration using a calibration curve generated by increasing amounts of sodium nitrite.
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