Fth1 rabbit polyclonal

Manufactured by Cell Signaling Technology

FTH1 rabbit polyclonal is a primary antibody product from Cell Signaling Technology. It targets the FTH1 protein, which functions as the heavy subunit of the ferratin complex.

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Lab products found in correlation

Lc3b rabbit polyclonal, by Cell Signaling Technology (1 mentions) P erk1 2 rabbit polyclonal, by Cell Signaling Technology (1 mentions) Sv40 ltag mouse monoclonal, by BD (1 mentions) Cyclin d1 rabbit polyclonal, by Santa Cruz Biotechnology (1 mentions) Pan actin rabbit polyclonal, by Cell Signaling Technology (1 mentions) Parp rabbit polyclonal, by Cell Signaling Technology (1 mentions)

2 protocols using fth1 rabbit polyclonal

Isolation and Immunoblotting of Cellular Proteins

Cited 1 time

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Protein isolation and Western blot analyses were performed according to previously published methods [7 (link)]. Primary antibodies were obtained from the following sources and used at the following dilutions: LC3B rabbit polyclonal (#2775S, 1:1000), p-ERK1/2 rabbit polyclonal (#9101S, 1:1000), total MAPK rabbit polyclonal (#4695S, 1:1000), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) rabbit polyclonal (#2118S, 1:4000), and FTH1 rabbit polyclonal (#3998S, 1:1000) were obtained from Cell Signaling Technology. CD71 mouse monoclonal (2B6, sc-51829, 1:100), TOM20 rabbit polyclonal (FL-145, sc-11415, 1:7500), TOM70 mouse monoclonal (A-8, sc-390545, 1:1000), IRP1 goat polyclonal (N-17, sc-14216, 1:1000) and IRP2 mouse monoclonal (7H6, sc-33682, 1:500) were obtained from Santa Cruz Biotechnology.

Bauckman K., Haller E., Taran N., Rockfield S., Ruiz-Rivera A, & Nanjundan M. (2015). Iron alters cell survival in a mitochondria-dependent pathway in ovarian cancer cells. Biochemical Journal, 466(Pt 2), 401-413.

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Western Blot Analysis of Cellular Proteins

Cited 2 times

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As previously described (24 (link), 25 (link)), proteins were extracted and normalized to at least 1500μg/ml. Proteins were subsequently analyzed on SDS-PAGE gels (8% or 10%, as appropriate) and transferred to polyvinylidene difluoride (PVDF) membranes for western blotting (24 (link), 25 (link)).
Primary antibodies that were utilized in this study are: SV40 LTAg mouse monoclonal (#554149, 1:1000, BD Biosciences), DNMT1 rabbit polyclonal (#5032, 1:1000, Cell Signaling Technology), HA-11 mouse monoclonal (#14921901, 1:500, Covance/Lab Corporation), CD71 mouse monoclonal (#sc-51829, 1:250, Santa Cruz Biotechnology), FTH1 rabbit polyclonal (#3998, 1:500, Cell Signaling Technology), pan-Actin rabbit polyclonal (#4968, 1:1000, Cell Signaling Technology), PARP rabbit polyclonal (#9542, 1:1000, Cell Signaling Technology), PAX-8 rabbit polyclonal (#10336-1-AP, 1:1000, Proteintech), Claudin-1 rabbit polyclonal (#187362, 1:10,000, Invitrogen), BAK rabbit monoclonal (#12105, 1: 1000, Cell Signaling Technology), Bcl-2 rabbit monoclonal (#2570, 1:1000, Cell Signaling Technology), Occludin-1 mouse monoclonal (#611090, 1:250, BD Biosciences), Cyclin D1 rabbit polyclonal (#sc-718, 1:1000, Santa Cruz Biotechnology), and Cyclin E mouse monoclonal (#sc-247, 1:500, Santa Cruz Biotechnology).

Chhabra R., Guergues J., Wohlfahrt J., Rockfield S., Espinoza Gonzalez P., Rego S., Park M.A., Berglund A.E., Stevens SM J.r, & Nanjundan M. (2023). Deregulated expression of the 14q32 miRNA cluster in clear cell renal cancer cells. Frontiers in Oncology, 13, 1048419.

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