Auditory brainstem response (ABR) hearing tests were performed using a TDT Auditory Workstation (Tucker-Davis Technologies) as previously described (Park et al., 2019 (link); Kim et al., 2019 (link)). Mice were anesthetized with ketamine (100 mg/kg) and xylazine (10 mg/kg) by intraperitoneal injection. Subdermal needle electrodes were placed at the vertex (active), ipsilateral ear (reference), and contralateral ear (ground). ABR thresholds were measured with a tone burst stimulus at 4, 8, 16, 32, 48, and 64 kHz. At each frequency, the sound level was reduced in 5–10 dB SPL steps from 90 to 10 dB SPL. A hearing threshold was determined as the lowest sound level that produced a noticeable ABR. Latencies and amplitudes for ABR wave I were also measured with a click stimulus of 100 dB peak SPL. A wave I latency was determined by measuring the amount of time elapsed from the onset of the stimulus to the highest value (peak) of the first ABR wave. A wave I amplitude was determined by measuring the voltage difference between the highest value (peak) and the lowest value (trough) of the first ABR wave. We used 6–10 mice per group for ABR threshold, wave I latency, and wave I amplitude measurements.
Tdt auditory workstation
Manufactured by Tucker-Davis Technologies
Sourced in Australia
The TDT Auditory Workstation is a comprehensive hardware and software suite designed for auditory research. It provides a flexible and integrated platform for presenting auditory stimuli, acquiring neural data, and analyzing experimental results. The workstation includes a range of hardware components, such as digital signal processors, analog-to-digital converters, and speaker drivers, as well as a software interface for experiment design and data management.
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3 protocols using tdt auditory workstation
1
Auditory Brainstem Response Measurement
2
Auditory Brainstem Response in Mice
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Mice were anaesthetised by intraperitoneal injection of ketamine (100 mg/kg) and xylazine (10 mg/kg), and placed into a sound-isolated chamber. Subcutaneous needle electrodes were inserted into the pinna and vertex, with a ground electrode near the hip. Responses to click stimuli and tone pip stimuli at 4, 8, 12, 24, and 32 kHz were recorded using a Power Lab 2/25 (AD Instruments, Sydney, Australia) and TDT Auditory Workstation (Tucker-Davis Technologies, Alachua, FL, USA). The duration of tone bursts was 1 msec. The sound level was raised in 5-dB steps from 0 dB to 100 dB. Overall, 500 responses were amplified and averaged. The threshold level was determined as the point above which any wave could be detected. All ABR were measured blinded to mouse genotype.
3
Auditory Brainstem Response Measurement in Mice
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The details of the ABR test and the method have been reported previously22 (link). We injected ketamine (100 mg/kg) and xylazine (10 mg/kg) into the peritoneal cavity of mice and put mice into a sound isolation chamber. Subcutaneous needle electrodes were inserted in the pinna and vertex, with a ground electrode near the tail. Responses to tone pip stimuli were recorded at 4, 8, 12, 24, and 32 kHz, intensities ranging from 0 to 100 dB-SPL instep of 5 dB, in 8–10-week-old mice using a Power Lab 2/25 (AD Instruments, Australia) and a TDT Auditory Workstation (Tucker-Davis Technologies, Alachua, Florida, USA). The duration of tone bursts was 1 ms. We amplified and averaged 500 responses. All ABRs were measured without knowing the profiles or genotypes of the mice.
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