Thiobarbituric acid tba

Manufactured by Loba Chemie

Sourced in India

Thiobarbituric acid (TBA) is a chemical compound used in various laboratory applications. It serves as a reagent for the detection and quantification of certain substances, particularly lipid peroxidation products. TBA is commonly utilized in analytical procedures to assess oxidative stress and related biological processes.

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Lab products found in correlation

Trichloroacetic acid tca, by Loba Chemie (2 mentions) L ascorbic acid, by Merck Group (1 mentions) Uv vis 1601, by Shimadzu (1 mentions) Dimethyl sulfoxide (dmso), by HiMedia (1 mentions) Arbutin, by Merck Group (1 mentions) Trypsin, by HiMedia (1 mentions) Dulbecco s modified eagle s medium, by HiMedia (1 mentions) Ethylenediaminetetraacetic acid, by HiMedia (1 mentions) Folin ciocalteu reagent, by Merck Group (1 mentions) Acetic anhydride, by Merck Group (1 mentions) 2 2 diphenyl 1 picrylhydrazyl (dpph), by Sisco Research Laboratories (1 mentions) Lead acetate, by Merck Group (1 mentions) Dragendorff reagent, by Merck Group (1 mentions) Trichloroaceticacid tca, by SD Fine Chemicals (1 mentions) Glacial acetic acid, by SD Fine Chemicals (1 mentions) Hydrogen peroxide, by SD Fine Chemicals (1 mentions) Chloroform, by SD Fine Chemicals (1 mentions) Wagner s reagent, by Merck Group (1 mentions) Ethyl acetate, by SD Fine Chemicals (1 mentions) Potassium chloride (kcl), by SD Fine Chemicals (1 mentions)

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Thiobarbituric acid (4 citations)

4 protocols using thiobarbituric acid tba

In vitro Anti-Lipid Peroxidation Assay

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In vitro determination of anti-lipid peroxidation assay was performed according to the method of Wills [26 (link)]. Briefly, the reaction mixtures contained 2.0 ml of the TCA-TBA-HCl reagent (15 % (w/v) Trichloroacetic Acid (TCA; Lot No# LM06461808; Loba Chemie), 0.375% (w/v) Thiobarbituric Acid (TBA; Lot No# SG53121901; Loba Chemie), and 0.25 N Hydrochloric Acid (HCl; CAS No# 7647-01-0; Fisher Chemical) and 1 ml of tests extracts of different concentrations (50, 100, 150 and 200 μg/ml) or standard (L-Ascorbic acid; CAS No# 21675-47-8; Sigma Aldrich, Germany). The resulting mixtures were incubated in a water bath (Labtech) set at 90 °C for 10 min, cooled, and Centrifuged at 10,000 rpm using a Labtech Centrifuge for 15 min. The supernatants were aspirated, and their respective absorbances measured at 532 nm using a double beam UV-Vis spectrophotometer (Shimadzu UV-Vis 1601).

Moriasi G.A., Ireri A.M., Nelson E.M, & Ngugi M.P. (2021). In vivo anti-inflammatory, anti-nociceptive, and in vitro antioxidant efficacy, and acute oral toxicity effects of the aqueous and methanolic stem bark extracts of Lonchocarpus eriocalyx (Harms.). Heliyon, 7(5), e07145.

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Arbutin Antioxidant Activity Assessment

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Arbutin (purity > 98 %) was procured from Sigma-Aldrich (USA) and prepared in 0.01 % dimethylsulfoxide (DMSO) (Himedia, India). Dulbecco's Modified Eagle's medium (DMEM), ethylenediaminetetraacetic acid (EDTA), foetal bovine serum (FBS), hydrogen peroxide, 3-(4,5-dimethylthiazole-2-yl)-2,5-dipenyltetrazolium bromide (MTT), nicotinamide adenine dinucleotide (NAD + and NADH) and trypsin were from Himedia; 5,5′-dithio-bis-2-nitrobenzoic acid (DTNB), glacial metaphosphoric acid, disodium hydrogen phosphate, manganese chloride (MnCl 2 ) and 2-mercaptoethanol were from SRL (India). Thiobarbituric acid (TBA) and trichloroacetic acid (TCA) were procured from Loba Chemie (India).

Tan J., Yadav M.K., Devi S, & Kumar M. (2022). Neuroprotective effects of arbutin against oxygen and glucose deprivation-induced oxidative stress and neuroinflammation in rat cortical neurons. Acta pharmaceutica (Zagreb, Croatia), 72(1).

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Thiobencarb Pesticide Formulation Analysis

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2.1. Technical thiobencarb (95%), and its traditional formulation, Saturn ® 50% EC were obtained from Kafr El-Zayat Company for Pesticides & Chemicals Production, Egypt. Acetylthiocholine iodide (ASChI), 5, 5'-dithiobis 2-nitrobenzoic acid (DTNB), sodium pyruvate, α-nicotinamide adenine dinucleotide (NADH), 1-Chloro 2, 4-dinitrobenzene (CDNB), 2amino-2-hydroxy methyl-propane-1, 3-diol (Tris-HCl), bovine serum albumin (BSA), and reduced glutathione (GSH) were supplied by Sigma Chem. Co. P.O. Box 14508 St. Louis MO 63178, USA. Sodium chloride (NaCl) and phosphate buffer, potassium phosphate monobasic; dibasic and potassium phosphate monobasic; dibasic were supplied by J.T. BAKER Chem. Co, Phillipsburg, N.J. 08865. Trichloroacetic acid (TCA; Cl3C3COOH) was obtained from Research Lab. Fine Chem. Indust., Mumbai 400002, India. Thiobarbituric acid (TBA) was supplied by LOBA CHEMIE PVT. Ltd, Mumbai-400005, India.

Abdel‐Halim K.Y., Aly G., Abdou G., & Oriby A. (2021). Potential toxic effects of nano-derived form of thiobencarb on two land snails, Theba pisana and Eobania vermiculata. Egyptian Journal of Chemistry, 0(0), 0-0.

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Phytochemical Extraction and Analysis

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Aluminum chloride, ascorbic acid, chloroform, ethanol, ethyl acetate, ferric chloride, ferrous sulfate, gallic acid, glacial acetic acid, hexane, hydrogen peroxide, α-naphthol, petroleum ether, potassium dihydrogen phosphate, potassium hydrogen phosphate, potassium chloride, potassium ferricyanide, sodium bicarbonate, trichloroacetic acid (TCA), and vanillin were procured from SD Fine Chemicals Limited, Mumbai, India. Acetone, hydrochloric acid, methanol, sodium hydroxide, and sulfuric acid were acquired from Spectrum Chemical Private Limited, Cochin. Acetic anhydride, Dragendorff reagent, Folin–Ciocalteu reagent, lead acetate, Mayer's reagent Sodium nitrate, and Wagner's reagent were purchased from MERCK Specialties Private Limited, Mumbai. 2,2-diphenyl-1-picrylhydrazyl (DPPH) was procured from SISCO Research Laboratories, Maharashtra, India. Tris base was from NR Chemicals Private Limited, Mumbai, India. Thiobarbituric acid (TBA) was bought from Loba Chemie Private Limited, Mumbai, India. All chemicals and reagents were of analytical grade.

Kamala A., Middha S.K., Gopinath C., Sindhura H.S, & Karigar C.S. (2018). In vitro Antioxidant Potentials of Cyperus rotundus L. Rhizome Extracts and Their Phytochemical Analysis. Pharmacognosy Magazine, 14(54), 261-267.

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