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Calcein am pi double staining kit

Manufactured by Merck Group
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Sourced in Japan

The Calcein-AM/PI double staining kit is a laboratory tool used to assess cell viability. It contains two fluorescent dyes, Calcein-AM and Propidium Iodide (PI), which differentially stain live and dead cells, respectively. Calcein-AM is cell-permeant and is converted to a green fluorescent dye in live cells, while PI only enters cells with compromised membranes, indicating cell death. The kit provides a simple and reliable method to distinguish and quantify live and dead cells in a sample.

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Calcein AM/PI staining kit Calcein-AM/PI Calcein AM staining Calcein-AM PI staining kit Calcein

2 protocols using calcein am pi double staining kit

1

Assessing HUVEC Viability in Hydrogel

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The Calcein-AM/PI double staining kit (Sigma-Aldrich) was applied to evaluate the viability of HUVECs cultured in RPMI 1640 with hydrogel extract liquid. Fluorescence images were acquired using a microscope (IX53, Olympus, Japan) at 24 h, 48 h and 72 h.
Zhang W., Zha K., Xiong Y., Hu W., Chen L., Lin Z., Yu C., Zhou W., Cao F., Hu H., Mi B, & Liu G. (2023). Glucose-responsive, antioxidative HA-PBA-FA/EN106 hydrogel enhanced diabetic wound healing through modulation of FEM1b-FNIP1 axis and promoting angiogenesis. Bioactive Materials, 30, 29-45.
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2

Cell Viability Evaluation of hBMSCs

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The Calcein-AM/PI double staining kit (Sigma-Aldrich) was applied to evaluate the viability of hBMSCs seeded on hydrogels for 96 h. Fluorescence images were acquired using a microscope (IX53, Olympus, Japan).
Lin Z., Xiong Y., Meng W., Hu Y., Chen L., Chen L., Xue H., Panayi A.C., Zhou W., Sun Y., Cao F., Liu G., Hu L., Yan C., Xie X., Lin C., Cai K., Feng Q., Mi B, & Liu G. (2021). Exosomal PD-L1 induces osteogenic differentiation and promotes fracture healing by acting as an immunosuppressant. Bioactive Materials, 13, 300-311.
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