The RNA oligoribonucleotides used in this study, including miR-1270 mimic, miR-1270 inhibitor, the small interfering RNAs (siRNAs) targeting CDR1as (si-CDR1as) or SCAI (si-SCAI), and the corresponding miRNA control (miR-NC) and siRNA control (si-NC), were purchased from GenePharma (Shanghai, China). The RNA oligoribonucleotides used in this study, including miR-1270 mimic, miR-1270 inhibitor, the siRNAs targeting CDR1as (si-CDR1as) or SCAI (si-SCAI), and the corresponding miRNA control (miR-NC) and siRNA control (si-NC), were purchased from GenePharma (Shanghai, China).
The lentivirus targeting human CDR1as was purchased from GeneChem (Shanghai, China). The targeting sequence was designed as follows: 1 sense 5′-TGCACCTGTGTCAAGGTCTTTTCAAGAGAAAGACCTTGACACAGGTGCTTTTTTC-3′ and 2 sense 5′-TGGTCTTCCAGCGACTTCAATTCAAGAGATTGAAGTCGCTGGAAGACCA-3′. miR-7 mimics and inhibitors were synthesized by RiboBio. The miRNA oligonucleotides were transfected using Lipofectamine RNAiMAX (50 nmol/L; Invitrogen, CA, USA).
The lentivirus targeting human CDR1as was purchased from GeneChem (Shanghai, China). The targeting sequence was designed as follows: 1 sense 5′-TGCACCTGTGTCAAGGTCTTTTCAAGAGAAAGACCTTGACACAGGTGCTTTTTTC-3′ and 2 sense 5′-TGGTCTTCCAGCGACTTCAATTCAAGAGATTGAAGTCGCTGGAAGACCA-3′. miR-7 mimics and inhibitors were synthesized by RiboBio. The miRNA oligonucleotides were transfected using Lipofectamine RNAiMAX (50 nmol/L; Invitrogen, CA, USA).