β actin and secondary antibodies

Gancaonin N was purchased from Chemfaces (Wuhan Chemfaces Biochemical Co., Ltd., Wuhan, China). LPS (Escherichia coli 055:B5) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Primary antibodies for COX-2, NF-κB p65, extracellular signal-regulated kinase (ERK), p-ERK, p38, p-p38, and Lamin B1 were purchased from Cell Signaling Technology (Beverly, MA, USA), interleukin (IL)-6, IL-1β, and tumor necrosis factor-α (TNF-α) were purchased from Proteintech (Rosemont, IL, USA), PGE2 was purchased from Bioss (Woburn, MA, USA), and iNOS was purchased from R&D Systems Inc. (Minneapolis, MN, USA). β-Actin and secondary antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

MCDB 131 medium, fetal bovine serum, L-glutamine, and epidermal growth factor (EGF) were purchased from Gibco (Grand Island, NY, USA). Prostaglandin E₂ (PGE₂), hydrocortisone, dimethyl sulfoxide (DMSO), and propidium iodide (PI) were purchased from Sigma-Aldrich (St. Louis, MO, USA), and human recombinant VEGF was obtained from ProSpec (Rehobot, Israel). Isookanin (purity ≥ 98%) was obtained from ChemFaces Biochemical Co. Ltd. (Wuhan, China) and dissolved in DMSO. Antibodies for phospho CREB and total CREB, phospho ERK1/2 and total ERK1/2, and cell cycle-related antibodies (Cyclin A₂, Cyclin B₁, Cyclin D₁) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA), while β-actin and secondary antibodies were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).