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Bs1074r fitc

Manufactured by Bioss Antibodies
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Sourced in United States

bs1074r-fitc is a fluorescently labeled antibody product offered by Bioss Antibodies. It is a research-use only tool designed for scientific applications.

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Lab products found in correlation

Bs 1091r cy5 5, by Bioss Antibodies (1 mentions) Nb100 78356g, by Novus Biologicals (1 mentions) Axio scope a1 microscope, by Zeiss (1 mentions) Tcs sp8, by Leica (1 mentions) Sc 66036, by Santa Cruz Biotechnology (1 mentions) Sc 516141, by Santa Cruz Biotechnology (1 mentions)

2 protocols using bs1074r fitc

1

Multiparametric Flow Cytometry Analysis

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Primary antibodies DyLight488-γH2AX (pSer139) (nb100-78356G NovusBio, Centennial, CO, USA), FITC-NRF2, (bs1074r-fitc, Bioss Antibodies, Inc. Woburn, MA, USA), FITC-BRCA1 (Nb100-598F, NovusBio, Centennial, CO, USA), PE-8-oxo-dG (sc-393871 PE, Santa Cruz Biotechnology, Dallas, TX, USA), CY5.5-NOX4 (bs-1091r-cy5-5, Bioss Antibodies, Inc. Woburn, MA, USA), A350-BCL2 (bs-15533r-a350, Bioss Antibodies, Inc. Woburn, MA, USA), BAX (Nb120-7977, NovusBio, Centennial, CO, USA) and secondary anti-rabbit IgG-FITC (sc-2359, Santa Cruz Biotechnology, Dallas, TX, USA) were used throughout.
Kostyuk S.V., Proskurnina E.V., Savinova E.A., Ershova E.S., Kraevaya O.A., Kameneva L.V., Umryukhin P.E., Dolgikh O.A., Kutsev S.I., Troshin P.A, & Veiko N.N. (2020). Effects of Functionalized Fullerenes on ROS Homeostasis Determine Their Cytoprotective or Cytotoxic Properties. Nanomaterials, 10(7), 1405.
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2

Immunofluorescent Imaging of Oxidative Stress Markers

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Culture cells and brain sections were incubated overnight with mouse monoclonal antibody against 8-oxodG (sc-66036, Santa Cruz) and NRF2 rabbit polyclonal antibody bs-1074R-FITC (BIOSS, USA). After washing with PBS with 1% BSA, they were incubated for 2 h (20°C) with m-IgGk BP-PE phycoerythrin conjugated (sc-516141, Santa Cruz) and then stained with DAPI. The antibody concentration was applied in accordance with the manufacturer's recommendations. Images were obtained using an AxioScope A1 microscope (Carl Zeiss) or confocal microscope (Leica TCS SP8).
Filev A.D., Shmarina G.V., Ershova E.S., Veiko N.N., Martynov A.V., Borzikova M.A., Poletkina A.A., Dolgikh O.A., Veiko V.P., Bekker A.A., Chirkov A.V., Volynshchikov Z.N., Deviataikina A.S., Shashin D.M., Puretskiy V.K., Tabakov V.J., Izhevskaya V.L., Kutsev S.I., Kostyuk S.V, & Umriukhin P.E. (2019). Oxidized Cell-Free DNA Role in the Antioxidant Defense Mechanisms under Stress. Oxidative Medicine and Cellular Longevity, 2019, 1245749.
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