Tissues were homogenized using a ThermoSavant FastPrep FP120 Homogenizer (Thermo Scientific, Wilmington, DE, USA) with Lysing MatrixD (MP Biomedicals, Illkirch, France). Total RNA was isolated using RNeasy Fibrous Tissue Mini Kit (QIAGEN GmbH, Hilden, Germany), quantified with NanoDrop 1000 (Thermo Scientific), and assessed by Agilent 2100 Bioanalyzer (Matriks AS, Oslo, Norway). Total RNAs were labeled, and Cy3-RNAs were fragmented and hybridized to the Whole Human Genome Oligo Microarray G4112A (Agilent Technologies, Santa Clara, CA, USA) on an Agilent scanner, and processed with Agilent Feature Extraction software v9.5 according to the manufacturer’s guidelines.
Thermosavant fastprep fp120 homogenizer
Manufactured by Thermo Fisher Scientific
Sourced in United States, United Kingdom
The ThermoSavant FastPrep FP120 Homogenizer is a laboratory equipment designed for the efficient disruption and homogenization of various biological samples, including cells, tissues, and microorganisms. It utilizes a high-speed reciprocating motion to effectively break down samples into a homogeneous suspension, enabling further downstream processing and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Nanodrop 1000, by Thermo Fisher Scientific (2 mentions)
Agilent feature extraction software v9, by Agilent Technologies (1 mentions)
Whole human genome oligo microarray g4112a, by Agilent Technologies (1 mentions)
Lysing matrix d, by MP Biomedicals (1 mentions)
Rneasy fibrous tissue mini kit, by Qiagen (1 mentions)
2 protocols using thermosavant fastprep fp120 homogenizer
1
Whole Genome Expression Profiling
2
Protein Extraction from Leaf Discs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For each construct used, 4–6 leaf discs were generated from infiltrated leaves using a sterile cork borer. The leaf discs were homogenized in three volumes of extraction buffer [50 mm Tris–HCl pH 7.25, 150 mm NaCl, 2 mm ethylenediaminetetraacetic acid (EDTA)] using ceramic beads in THERMO® Savant FastPrep FP120 Homogenizer (Thermo Fischer Scientific, Loughborough, UK). The homogenate was clarified by centrifugation for 15 min at 16 000 g using a cold bench-top centrifuge. The supernatant represented the total soluble protein extract.
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