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Anti pdgfrβ

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Anti-PDGFRβ is a laboratory reagent used for the detection and analysis of PDGFRβ (platelet-derived growth factor receptor beta) in biological samples. It is a primary antibody that specifically binds to the PDGFRβ protein, allowing researchers to study its expression and distribution in cells and tissues.

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Lab products found in correlation

Ripa lysis buffer, by Thermo Fisher Scientific (2 mentions) Anti rb, by Cell Signaling Technology (1 mentions) Nupage bis tris gel, by Thermo Fisher Scientific (1 mentions) Protease inhibitor, by Thermo Fisher Scientific (1 mentions) Anti p27kip1, by Cell Signaling Technology (1 mentions) Anti hsp70, by Proteintech (1 mentions) Enhanced chemi luminescence (ecl), by Thermo Fisher Scientific (1 mentions) Anti prb, by Cell Signaling Technology (1 mentions) Anti γh2ax, by Cell Signaling Technology (1 mentions) Anti cleaved parp, by Cell Signaling Technology (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Anti p21, by Cell Signaling Technology (1 mentions) Anti rabbit igg hrp, by Cell Signaling Technology (1 mentions) Anti mouse igg hrp, by Cell Signaling Technology (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions) Anti stat1, by Cell Signaling Technology (1 mentions) Anti stat2, by Cell Signaling Technology (1 mentions) Anti phospho stat3 y705, by Cell Signaling Technology (1 mentions) Anti tyk2, by Cell Signaling Technology (1 mentions) Anti jak2, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

PDGFRβ (3 citations) Anti-TM (2 citations)

3 protocols using anti pdgfrβ

1

Western Blot Analysis of Cellular Proteins

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Protein was extracted from cells cultured in 6-well plates using RIPA lysis buffer (ThermoFisher, 89900) with protease inhibitors (ThermoFisher, 78440). A total of 30 μg protein lysates was separated by 4-12% NuPage Bis-Tris gels (ThermoFisher, NP0336) and then transferred to polyvinylidene fluoride (PVDF) membranes (BioRad, 1620264). The membranes were blocked with 5% Bovine Serum Albumin (BSA) (Sigma-Aldrich, A7906-100G), probed with primary antibodies overnight at 4 °C, and then incubated with the relevant secondary antibody for 1 hour at room temperature. Bands were visualized using ECL (ThermoFisher, 34080) with BioRad imaging system. The antibodies included anti-cleaved-PARP (Cell Signaling Technologies, 5625), anti-p21 (Cell Signaling Technologies, 2947), anti- p27kip1 (Cell Signaling Technologies, 3686), anti-Rb (Cell Signaling Technologies, 9309), anti-p-Rb (Cell Signaling Technologies, 8516), anti-AXL (Proteintech, 13196-1-AP), anti-ATRX (Novus Biologicals, NBP1-32851), anti-PDGFRβ (Proteintech, 13449-1-AP), anti-HSP70 (Proteintech, 10995-1-AP), anti-γH2AX (Cell Signaling Technologies, 9718T), anti-β-actin (Cell Signaling Technologies, 12620S), anti-rabbit-IgG-HRP (Cell Signaling Technologies, 7074S), and anti-mouse-IgG-HRP (Cell Signaling Technologies, 7076S) per instruction from the data sheet.
Yu S., Wei S., Savani M., Lin X., Du K., Mender I., Siteni S., Vasilopoulos T., Reitman Z.J., Ku Y., Wu D., Liu H., Tian M., Chen Y., Labrie M., Charbonneau C.M., Sugarman E., Bowie M., Hariharan S., Waitkus M., Jiang W., McLendon R.E., Pan E., Khasraw M., Walsh K.M., Lu Y., Herlyn M., Mills G., Herbig U., Wei Z., Keir S.T., Flaherty K., Liu L., Wu K., Shay J.W., Abdullah K., Zhang G, & Ashley D.M. (2021). A Modified Nucleoside 6-thio-2’-deoxyguanosine Exhibits Anti-tumor Activity in Gliomas. Clinical cancer research : an official journal of the American Association for Cancer Research, 27(24), 6800-6814.
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2

Immunoblotting for PDGF-stimulated Signaling

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Antibodies used were anti-Abi1 (1:1000, Sigma #A5106-200UL, L/N 076M4842V), anti-GAPDH (1: 1000, Santa Cruz Biotechnology #sc-32233, K0315), anti-JAK2 (Santa Cruz Biotechnology #sc-294, G1414 and Thermo# AHO1352, QC216934), anti-phospho-Jak2 (Y1007/Y1008) (Cell Signaling, #3771S/10), anti-phospho-Jak1 (Y1034/Y1035) (Cell Signaling, # 74129S/2), anti-Jak1 (Cell Signaling, #50996), anti-phospho-Jak3 (Y980/Y981) (Cell Signaling, # 5031S/7), anti-Jak3 (Cell Signaling, #5481), anti-phospho-Tyk2 ((Y1054/Y1055) (Cell Signaling, # 68790S/1), anti-Tyk2 (SC-5271), anti-phospho-STAT1 (Y701) (Cell Signaling, #9167S/25), anti-STAT1 (Cell Signaling, #9176S), anti-phospho-STAT2 (Y960) (Cell Signaling, # 88410S/4), anti-STAT2 (Cell Signaling, #4597S), anti-phospho-Stat3 (Y705) (Cell Signaling, #9145/1), anti-Stat3 (Invitrogen, #MA1-13042/PJ208446), anti-phospho-PDGFRβ (Tyr751) (Cell Signaling, #3161/7) and anti-PDGFRβ (Proteintech, #13449-1-AP/# 00070807). The antibodies were validated by examining the molecular weight of target proteins. In addition, anti-Abi1 was validated by using Abi1 KD cells. Finally, vendors have provided datasheet to show that antibodies were validated by positive controls. For protein phosphorylation experiments, cells were treated with 10 ng/mL PDGF for 10 min followed by immunoblot analysis (see below).
Wang R., Wang Y., Liao G., Chen B., Panettieri RA J.r., Penn R.B, & Tang D.D. (2022). Abi1 mediates airway smooth muscle cell proliferation and airway remodeling via Jak2/STAT3 signaling. iScience, 25(2), 103833.
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3

Western Blot Analysis of Tight Junction Proteins

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Cortex was extracted, lysed with RIPA buffer (Beyotime, P0013B) containing phosphatase inhibitor cocktail (Invitrogen, 78443), and a Pierce™ BCA Protein Assay Kit (Invitrogen, 23225) was used to quantify the protein concentration using a microplate reader. Samples were loaded and separated on 7.5% and 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (EpiZyme, PG110-112, CHN), and transferred to polyvinylidene uoride (PVDF) membranes (Millipore) according to the molecular weight of targeted protein. After blockage by 5% non-fat milk for 1 h, membranes were incubated overnight with primary antibodies, including Anti-GAPDH (Abcam, 1:1000, ab8245, ab9425), Anti-ZO-1 (A nity Biosciences, 1:1000, AF5145), Anti-Occludin (A nity Biosciences, 1:1000, DF7504), Anti-Claudin-5 (A nity Biosciences, 1:1000, AF5216), Anti-PDGF-BB (A nity Biosciences, 1:1000, AF0240), Anti-PDGFR-β (Proteintech Group, 1:1000, 13449-1-AP), followed by incubation with appropriate HRP-conjugated secondary antibodies (1:3000) at room temperature for 1 h. An Image Quant LAS 4000 detection system (GE Healthcare Life Science, Chicago, USA) was used to visualize the target proteins on the membranes. Protein expression levels were normalized to GAPDH and quanti ed using Image J software.
Li W., Niu X., Dai Y., Wu X., Li J, & Sheng W. (2023). Rnf-213 Knockout Induces Pericyte Reduction and Blood-Brain Barrier Impairment in Mouse. Molecular neurobiology, 60(11).
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