Cells (Nup96-SNAP-tag, catalog no. 300444, CLS Cell Line Service, Eppelheim, Germany) were permeabilized and fixed as described for microtubule staining. Successive blocking was done for 30 min in 2% (w/v) BSA in PBS and 30 min in Image-iT FX Signal Enhancer before the sample was incubated in a mixture of rabbit antibodies against clathrin light chain (diluted 1:100, catalog no. sc-28276, Santa Cruz Biotechnology) and clathrin heavy chain (diluted 1:300, catalog no. ab21679, abcam) in 1% w/v BSA in PBS overnight at 4 °C. Unbound antibodies were washed away 3x 5 min in PBS before the sample was stained with secondary anti rabbit antibody coupled to Dy643 (diluted 1:300 in PBS containing 1% (w/v) BSA, inhouse made by NHS ester coupling of Dy634 to catalog no. 711-005-152, Dianova, Hamburg, Germany) for 3 h at RT. Microtubules were further stained with AF647, as described previously. The sample was washed 3x 5 min with PBS, before mounting it onto a custom manufactured sample holder in appropriate imaging buffer (see section Imaging buffers). The holder was sealed with parafilm.
Sc 28276
Manufactured by Santa Cruz Biotechnology
Sc-28276 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is a compact and versatile device designed for general laboratory use. The core function of Sc-28276 is to facilitate various experimental procedures and data collection tasks commonly performed in research and testing environments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sc 28276
1
Clathrin and Microtubule Staining Protocol
2
Clathrin and Microtubule Staining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells (Nup96-SNAP-tag, catalog no. 300444, CLS Cell Line Service, Eppelheim, Germany) were permeabilized and fixed as described for microtubule staining. Successive blocking was done for 30 min in 2% (w/v) BSA in PBS and 30 min in Image-iT FX Signal Enhancer before the sample was incubated in a mixture of rabbit antibodies against clathrin light chain (diluted 1:100, catalog no. sc-28276, Santa Cruz Biotechnology) and clathrin heavy chain (diluted 1:300, catalog no. ab21679, abcam) in 1% w/v BSA in PBS overnight at 4 °C. Unbound antibodies were washed away 3x 5 min in PBS before the sample was stained with secondary anti rabbit antibody coupled to Dy643 (diluted 1:300 in PBS containing 1% (w/v) BSA, inhouse made by NHS ester coupling of Dy634 to catalog no. 711-005-152, Dianova, Hamburg, Germany) for 3 h at RT. Microtubules were further stained with AF647, as described previously. The sample was washed 3x 5 min with PBS, before mounting it onto a custom manufactured sample holder in appropriate imaging buffer (see section Imaging buffers). The holder was sealed with parafilm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!