VIP was purchased from Nanjing Peptide Biotechnology Inc. (Nanjing, China). Fetal bovine serum (FBS), a modification of Minimum Essential Medium (a-MEM), and L-glutamine were supplied by Invitrogen (Carlsbad, USA). Oligonucleotide primers were ordered from Sangon Biotech (Shanghai, China). TaqMan Universal PCR Master Mix and probes were obtained from Applied Biosystems (Foster City, CA). anti-RANKL, anti-RANK, anti-OPG, anti-NF-κB, anti-interleukin 6 (IL-6), anti-ERK, anti-carbonic anhydrase II (CAII), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) primary, and anti-IgG-horseradish peroxidase secondary antibodies were purchased from Bioss Biotechnology, Inc. (Beijing, China).
Anti rank
Manufactured by Bioss Antibodies
Sourced in China
Anti-RANK is an antibody that binds to the Receptor Activator of Nuclear Factor-κB (RANK) protein. RANK is a cell surface receptor that plays a key role in the regulation of bone metabolism, immune response, and other cellular processes. This antibody can be used for the detection and study of RANK in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti rankl, by Bioss Antibodies (2 mentions)
Anti opg, by Bioss Antibodies (1 mentions)
Anti nf κb, by Bioss Antibodies (1 mentions)
Anti erk, by Bioss Antibodies (1 mentions)
Taqman universal pcr master mix and probes, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Oligonucleotide primers, by Sangon (1 mentions)
C digit blot scanner, by LI COR (1 mentions)
Anti bcl 2, by Santa Cruz Biotechnology (1 mentions)
Anti β tubulin antibody, by Elabscience (1 mentions)
Ripa lysis buffer, by Merck Group (1 mentions)
Anti bax, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti rank
1
VIP-Induced Osteoclastogenesis Regulation
2
Protein Expression Analysis of RANK-RANKL-OPG Axis
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Proteins were extracted from treated and non-treated cells using RIPA Lysis Buffer (Millipore) and following the manufacturer’s instructions. RANK, RANK-L, OPG, pCDK2, BAX, Bcl-2 proteins were characterized in total lysates from cell line cultures by Western blotting. Membranes were incubated overnight at 4°C with the following antibodies: anti-RANK L (1:100, BIOSS), anti-RANK (1:250, BIOSS), anti-OPG (1:500, Elabscience), anti-pCDK2 (1:1000, abcam), anti-BAX (1:200, Santa Cruz), anti-Bcl2 (1:100, Santa Cruz). Reactive bands were detected by chemiluminescence (Immobilon western Millipore) on a C-DiGit® Blot Scanner (LI-COR Biosciences). A mouse monoclonal anti-β-Tubulin antibody (1:5000, Elabscience) was used to check for comparable protein loading and as a housekeeping protein. Images were captured, stored and analyzed using “Image studio Digits ver. 5.0” software.
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