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Alex fluor 594 green conjugated secondary antibody

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The Alexa Fluor 594 (Green) conjugated secondary antibody is a fluorescent-labeled antibody used in immunoassays and imaging applications. It binds to the Fc region of primary antibodies, allowing for the detection and visualization of target proteins or molecules.

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Lab products found in correlation

Phenyl methyl sulfonyl fluoride (pmsf), by Sangon (2 mentions) Fluorescence optical microscope, by Leica (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Fluorescence microscopy, by Olympus (1 mentions) Formaldehyde, by Sangon (1 mentions)

Spelling variants of this product

Alex Fluor 594 (10 citations) Alex-594 (5 citations)

3 protocols using alex fluor 594 green conjugated secondary antibody

1

Immunofluorescence Staining of Flag-Tagged Cells

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Cells were washed with cold PBS and fixed with 4% Formaldehyde (Sangon) for 15 min at room temperature. Then, cells were treated with 0.3% Triton X-100 for cell perforation at room temperature for 15 min, and were incubated with blocking buffer (3% BSA in PBS) for 1 h, followed by incubation at 4 °C overnight with the primary antibody against Flag, and Alex Fluor 594 (Green) conjugated secondary antibody (Invitrogen) at room temperature for 1 h. Cell nucleus was stained with DAPI (Invitrogen). The cells were examined by fluorescence microscopy (Olympus America Inc, Center Valley, PA).
Xia Y.K., Zeng Y.R., Zhang M.L., Liu P., Liu F., Zhang H., He C.X., Sun Y.P., Zhang J.Y., Zhang C., Song L., Ding C., Tang Y.J., Yang Z., Yang C., Wang P., Guan K.L., Xiong Y, & Ye D. (2020). Tumor-derived neomorphic mutations in ASXL1 impairs the BAP1-ASXL1-FOXK1/K2 transcription network. Protein & Cell, 12(7), 557-577.
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2

Immunofluorescent Assay for γH2AX Detection

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Cells were washed with cold PBS and fixed with 4% PMSF (Sangon) for 15 min at room temperature. Then, cells were treated with 0.3% Triton X-100 for cell perforation at room temperature for 15 min, and were incubated with blocking buffer (3% BSA in PBS) for 1 hour, followed by incubation at 4°C overnight with the primary antibody against γH2AX, and Alex Fluor 594 (Green) conjugated secondary antibody (Invitrogen) at room temperature for 1 hour. Cell nucleus was stained with DAPI (Invitrogen). Images were captured using Leica fluorescence optical microscope.
Chen L.L., Lin H.P., Zhou W.J., He C.X., Zhang Z.Y., Cheng Z.L., Song J.B., Liu P., Chen X.Y., Xia Y.K., Chen X.F., Sun R.Q., Zhang J.Y., Sun Y.P., Song L., Liu B.J., Du R.K., Ding C., Lan F., Huang S.L., Zhou F., Liu S., Xiong Y., Ye D, & Guan K.L. (2018). SNIP1 Recruits TET2 to Regulate c-MYC Target Genes and Cellular DNA Damage Response. Cell reports, 25(6), 1485-1500.e4.
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3

Immunofluorescent Assay for γH2AX Detection

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Cells were washed with cold PBS and fixed with 4% PMSF (Sangon) for 15 min at room temperature. Then, cells were treated with 0.3% Triton X-100 for cell perforation at room temperature for 15 min, and were incubated with blocking buffer (3% BSA in PBS) for 1 hour, followed by incubation at 4°C overnight with the primary antibody against γH2AX, and Alex Fluor 594 (Green) conjugated secondary antibody (Invitrogen) at room temperature for 1 hour. Cell nucleus was stained with DAPI (Invitrogen). Images were captured using Leica fluorescence optical microscope.
Chen L.L., Lin H.P., Zhou W.J., He C.X., Zhang Z.Y., Cheng Z.L., Song J.B., Liu P., Chen X.Y., Xia Y.K., Chen X.F., Sun R.Q., Zhang J.Y., Sun Y.P., Song L., Liu B.J., Du R.K., Ding C., Lan F., Huang S.L., Zhou F., Liu S., Xiong Y., Ye D, & Guan K.L. (2018). SNIP1 Recruits TET2 to Regulate c-MYC Target Genes and Cellular DNA Damage Response. Cell reports, 25(6), 1485-1500.e4.
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