Sigma rpmi 1460 hepes modified culture medium

Manufactured by Merck Group

Sigma RPMI 1460 HEPES modified culture medium is a commonly used cell culture medium formulation. It is a modification of the Roswell Park Memorial Institute (RPMI) 1640 medium, which includes the addition of the HEPES buffer system. This medium is designed to support the growth and maintenance of a variety of cell types in in vitro cell culture applications.

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RPMI-1460 medium RPMI- HEPES modified RPMI 1460 Modified RPMI medium RPMI culture medium RPMI medium HEPES

2 protocols using sigma rpmi 1460 hepes modified culture medium

Establishing Patient-Derived Ovarian Cancer Cultures

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Ethical approval was granted (12/NW/0202) for the collection of ascites from consented patients undergoing surgery for EOC at the Queen Elizabeth Hospital, Gateshead, UK. Clinical details were recorded and specimens registered and handled in accordance with the Human Tissue Act. Samples were assigned a reference number to retain anonymity.
PCO cultures were generated and maintained as previously described [39 (link), 40 (link)]. Briefly 20ml of ascites was added to 20ml of warmed Sigma RPMI 1460 HEPES modified culture medium supplemented with 20% v/v fetal calf serum and 100µl/ml penicillin and streptomycin in T75 flasks and incubated at 37°C, 5% CO₂ humidified air.

McCormick A., Donoghue P., Dixon M., O’Sullivan R., O’Donnell R.L., Murray J., Kaufmann A., Curtin N.J, & Edmondson R.J. (2016). Ovarian Cancers Harbour Defects in Non-Homologous End Joining Resulting in Resistance to Rucaparib. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(8), 2050-2060.

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Establishment of Primary Ovarian Cancer Cultures

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Ethical approval was granted (12/NW/0202) for the collection of ascites from consented patients undergoing surgery for ovarian cancer at the Queen Elizabeth Hospital, Gateshead, UK. Clinical details were recorded and specimens registered and handled in accordance with the Human Tissue Act. Samples were assigned a reference number to retain anonymity. Histopathology and tumour grades were assigned by a pathologist according to the International Federation of Gyneacology and Obstetrics (FIGO) criteria. Primary cultures were generated and maintained as previously described [10 (link)]. Briefly 20 ml of ascites was added to 20 ml of warmed Sigma RPMI 1460 HEPES modified culture medium supplemented with 20% v/v foetal calf serum and 100 μl/ml penicillin and streptomycin in T75 flasks and incubated at 37°C, in 5% CO₂ humidified air. Cells were passaged, frozen and thawed as previously described [14 (link)].

McCormick A., Earp E., Elliot K., Cuthbert G., O'Donnell R., Wilson B.T., Sutton R., Leeson C., Thomas H.D., Blair H., Fordham S., Lunec J., Allan J, & Edmondson R.J. (2017). Functional characterisation of a novel ovarian cancer cell line, NUOC-1. Oncotarget, 8(16), 26832-26844.

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