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Nitrocellulose membrane disc

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The Nitrocellulose membrane disc is a widely used laboratory product designed for various filtration and immobilization applications. It is a thin, porous membrane made of nitrocellulose material that allows the passage of small molecules and particles while retaining larger molecules or particles on the surface. The core function of the nitrocellulose membrane disc is to facilitate efficient separation, capture, and immobilization of biomolecules, proteins, and other analytes during various analytical and experimental procedures.

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Lab products found in correlation

Ames medium, by Merck Group (2 mentions) Ames solution, by Merck Group (1 mentions)

Close spelling variants of this product

Nitrocellulose membranes Nitrocellulose

3 protocols using nitrocellulose membrane disc

1

Mouse Retina Isolation and Preparation

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Mice were deeply euthanized with isoflurane followed by decapitation, and their eyes were enucleated. For immunohistochemistry and biolistic transfection, the eyes were then transferred to oxygenated mouse artificial cerebrospinal fluid (mACSF, pH 7.4) containing (in μm) 119 NaCl, 2.5 KCl, 2.5 CaCl2, 1.3 MgCl2, 1 NaH2PO4, 11 glucose and 20 HEPES at room temperature. After removing the anterior eye and vitreous, a deep cut was made on ventral side to mark the orientation of the eyes (Wei et al., 2010 (link)). The retina was isolated from the eyecup and mounted on a nitrocellulose membrane disc (Millipore) with retinal ganglion cell side up. The retinas were subsequently fixed for 15–20 minutes in 4% paraformaldehyde in mACSF. For intracellular dye-fill, the dorsal pole of the left and right eyes were marked before removing them from the animal, using waterproof color markers thus ensuring that knowledge about retinal location was preserved (Wei et al., 2010 (link)). Retinas were isolated and kept in oxygenated (95% O2/5% CO2) NaHCO3 (23 μm) containing Ames’ medium (Sigma-Aldrich).
Yu W.Q., El-Danaf R.N., Okawa H., Pacholec J.M., Matti U., Schwarz K., Odermatt B., Dunn F.A., Lagnado L., Schmitz F., Huberman A.D, & Wong R.O. (2018). Synaptic Convergence Patterns onto Retinal Ganglion Cells Are Preserved despite Topographic Variation in Pre- and Postsynaptic Territories. Cell reports, 25(8), 2017-2026.e3.
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2

Mouse Retina Isolation and Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were deeply euthanized with isoflurane followed by decapitation, and their eyes were enucleated. For immunohistochemistry and biolistic transfection, the eyes were then transferred to oxygenated mouse artificial cerebrospinal fluid (mACSF, pH 7.4) containing (in μm) 119 NaCl, 2.5 KCl, 2.5 CaCl2, 1.3 MgCl2, 1 NaH2PO4, 11 glucose and 20 HEPES at room temperature. After removing the anterior eye and vitreous, a deep cut was made on ventral side to mark the orientation of the eyes (Wei et al., 2010 (link)). The retina was isolated from the eyecup and mounted on a nitrocellulose membrane disc (Millipore) with retinal ganglion cell side up. The retinas were subsequently fixed for 15–20 minutes in 4% paraformaldehyde in mACSF. For intracellular dye-fill, the dorsal pole of the left and right eyes were marked before removing them from the animal, using waterproof color markers thus ensuring that knowledge about retinal location was preserved (Wei et al., 2010 (link)). Retinas were isolated and kept in oxygenated (95% O2/5% CO2) NaHCO3 (23 μm) containing Ames’ medium (Sigma-Aldrich).
Yu W.Q., El-Danaf R.N., Okawa H., Pacholec J.M., Matti U., Schwarz K., Odermatt B., Dunn F.A., Lagnado L., Schmitz F., Huberman A.D, & Wong R.O. (2018). Synaptic Convergence Patterns onto Retinal Ganglion Cells Are Preserved despite Topographic Variation in Pre- and Postsynaptic Territories. Cell reports, 25(8), 2017-2026.e3.
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3

Retinal Tissue Preparation Protocols

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For immunohistochemistry, biolistic transfection, and live-cell imaging, mice were deeply euthanized with isoflurane, decapitated, and enucleated. Retinas were then dissected in oxygenated mouse artificial cerebrospinal fluid (mACSF, pH 7.4) containing (in mM) 119 NaCl, 2.5 KCl, 2.5 CaCl2, 1.3 MgCl2, 1 NaH2PO4, 11 glucose, and 20 HEPES at room temperature. The retina was isolated from the eyecup first and mounted on a nitrocellulose membrane disc (Millipore) with retinal ganglion cell side up. For electrophysiology, mice were dark-adapted overnight and sacrificed by cervical dislocation under infrared illumination. The retina was mounted photoreceptor side down on poly-lysine coated cover slides (BD Biosciences) and perfused with oxygenated Ames solution (Sigma) heated at 32 °C.
Okawa H., Yu W.Q., Matti U., Schwarz K., Odermatt B., Zhong H., Tsukamoto Y., Lagnado L., Rieke F., Schmitz F, & Wong R.O. (2019). Dynamic assembly of ribbon synapses and circuit maintenance in a vertebrate sensory system. Nature Communications, 10, 2167.
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