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3 protocols using sodium dihydrogen phosphate dodecahydrate

1

Lanthanide-Doped Upconversion Nanoparticles

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Hexaammonium molybdate tetrahydrate ((NH4)6Mo7O24·4H2O), sodium dihydrogen phosphate dodecahydrate (NaH2PO4·2H2O), hydrochloric acid (HCl), Er2O3 (99.99%), Nd2O3 (99.99%), Gd2O3 (99.99%), Yb2O3 (99.99%) and sodium fluoride (NaF) were acquired from the Sinopharm Chemical Reagent Co., Ltd. Oleic acid (OA), 3-4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide (MTT), 1-octadecene (ODE), 4′,6-diamidino-2-phenylindole (DAPI), l-ascorbic acid, disodium maleate, doxorubicin (DOX), propidium iodide (PI), calcein AM, and methoxy PEG silane (Mw = 2000) were acquired from Sigma-Aldrich. Tetraethyl orthosilicate (TEOS), cetyltrimethyl ammonium bromide (CTAB), 3-aminopropyltriethoxysilane (APTES) and ammonium nitrate (NH4NO3) were acquired from the Tianjin Kermel Chemical Reagent Co., Ltd. Manganese(ii) sulfate monohydrate (MnSO4·H2O), trifluoroacetic acid (CF3COOH), and sodium trifluoroacetate (CF3COONa) were acquired from the Beijing Chemical Reagent Co. All of the chemical reagents used in this work were of analytical grade without further purification.
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2

Enzymatic Catalysis in Biomaterials

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Citric acid and PEPA were purchased from the Aladdin Industrial Corporation (Shanghai, China). Tyrosinase (TYR) from mushrooms, DA, GSH, ascorbic acid (AA), glucose oxidase (GOD), alkaline phosphatase (ALP), glycine (Gly), lysozyme, trypsin and catalase were supplied by Sigma-Aldrich. Cysteine, acetone, sodium chloride, sodium dihydrogen phosphate dodecahydrate (Na2HPO4·12H2O), sodium phosphate monobasic dihydrate (NaH2PO4·2H2O) and glucose were obtained from Sinopharm Chemical Reagent Co., Ltd. All aqueous solutions used in this work were prepared in ultrapure water obtained from a Millipore system. All analytical grade reagents were used without any purification.
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3

Antioxidant Evaluation of Oysters

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The same batch of frozen oysters from South China Sea, with good commodity quality were obtained from Raoping Yujie Food Industry Co., Ltd. (Guanngdong, China), transported to the laboratory through a cold chain, stored at −20 °C, then transferred to 4 °C the night before the experiment for defrosting. RO (the content of carnosic acid was 15.36%, w/w) was obtained from Kalsec Co, Ltd. (Shanghai, China). Fish 4-HNE ELISA Kit was obtained from Beijing Kangpu Tongchuang Co, Ltd. (Beijing, China). 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) were obtained from Macklin Regent Co, Ltd. (China). Trichloroacetic acid (TCA) was from Shanghai Aladdin Reagent Inc. (Shanghai, China). Sodium dihydrogen phosphate dihydrate, 2-thiobarbituric acid (TBA), absolute ethanol (analytical reagent), Tween-80, and sodium dihydrogen phosphate dodecahydrate (Sinopharm Chemical Regent Co, Ltd., Beijing, China) were used.
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