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Renilla reniformis

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Renilla reniformis is a bioluminescent marine invertebrate that produces a luciferase enzyme. This enzyme can be used as a reporter in various biological assays and applications.

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Lab products found in correlation

Top fop flash, by Genechem (3 mentions) Top fop flash, by Promega (3 mentions) Dual luciferase reporter assay, by Promega (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Dual luciferase reporter assay system, by Promega (1 mentions)

Close spelling variants of this product

Renilla reniformis luciferase reporter vector (4 citations) Renilla reniformis luciferase (4 citations) PRL-TK™ Renilla reniformis luciferase plasmid (2 citations) Photinus pyralis-Renilla reniformis dual luciferase reporter assay system (2 citations)

4 protocols using renilla reniformis

1

WNT2B Regulation by LINC00665

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TOP/FOP‐Flash (Genechem) was co‐transfected into cells (5 × 105) along with the transfection of sh‐NC, sh‐LINC00665 or sh‐LINC00665 + pcDNA‐WNT2B. The TOP/FOP‐Flash values were normalized to the Renilla reniformis (Promega).
Bai J., Zhang X., Jiang F., Shan H., Gao X., Bo L, & Zhang Y. (2022). A Feedback Loop of LINC00665 and the Wnt Signaling Pathway Expedites Osteosarcoma Cell Proliferation, Invasion, and Epithelial‐Mesenchymal Transition. Orthopaedic Surgery, 15(1), 286-300.
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2

Wnt Signaling Pathway Activation Assay

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Briefly, tumor cells (5 × 104) were seeded into a 24-well plate, and TOP-Flash reporter plasmids and pTK-RL plasmids were transiently co-transfected into the cells using Lipofectamine 2000 (Invitrogen). After transfection for 48 hours, the Dual-Luciferase Reporter Assay (Promega) was applied according to the manufacturer’s instructions. For the TOP/FOP-Flash assay, TOP/FOP-Flash (Genechem) was co-transfected into cells along with HNRNPM depletion or overexpression, HNRNPM-ASO, MBD2a silence, FZD3 silence, or MBD2c overexpression, and/or control. The TOP/FOP-Flash values were normalized to the Renilla reniformis (Promega) reading and the TOP/FOP ratio was measured. Experiments were performed in triplicate.
Zhu G.Q., Wang Y., Wang B., Liu W.R., Dong S.S., Chen E.B., Cai J.L., Wan J.L., Du J.X., Song L.N., Chen S.P., Yu L., Zhou Z.J., Wang Z., Zhou J., Shi Y.H., Fan J, & Dai Z. (2022). Targeting HNRNPM Inhibits Cancer Stemness and Enhances Antitumor Immunity in Wnt-activated Hepatocellular Carcinoma. Cellular and Molecular Gastroenterology and Hepatology, 13(5), 1413-1447.
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3

Quantifying Wnt Pathway Activity

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A TOPFlash construct (400 ng) and a Renilla reporter (400 ng) were separately mixed with a TK control reporter (50 ng) and cotransfected into 106 cells by electroporation. For pGLMV-Gcm1 transfection for Gcm1 overexpression, PGLMV-Gcm1 (4 µg), TOPFlash construct, FOPFlash construct and TK control construct were all mixed together. After 72 h, the Dual-Luciferase® Reporter Assay System (Cat #E2920, Promega, USA) was used to measure the luciferase activity according to the manufacturer’s protocol. The TOP/FOP-flash values were normalized to the Renilla reniformis (Promega, USA) reading and the TOP/FOP ratio was measured.
Li J., Xie Q., Gao J., Wang F., Bao Y., Wu L., Yang L., Liu Z., Guo R., Khan A., Dan L.i.u., Li C., Wu J, & Xie J. (2021). Aberrant Gcm1 expression mediates Wnt/β-catenin pathway activation in folate deficiency involved in neural tube defects. Cell Death & Disease, 12(3), 234.
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4

Dual-Luciferase Assay for miRNA-LUCAT1 Regulation

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Full-length LUCAT1 sequence of wild-type (WT) and mutant-type (MUT) miRNA binding site was constructed in Genechem Co., Ltd. (Shanghai, China). LUCAT1 WT/MUT were transfected into MCF-7 with miR-5582-3p mimic/NC mimic. Similarly, the binding sites for miR-5582-3p in the 3′-untranslated region (3′-UTR) sequence of TCF7L2 were obtained from Genechem. TCF7L2 3′-UTR WT/MUT were transfected into MCF-7 with miR-5582-3p mimic/NC mimic. The Dual-Luciferase Reporter Assay (Promega) was applied according to the manufacturer’s instructions. For the TOP/FOP-Flash assay, TOP/FOP-Flash (Genechem) was co-transfected into cells along with LUCAT1 silence or overexpression vector, miR-5582-5p mimic or inhibitor, and/or the miRNA control. The TOP/FOP-Flash values were normalized to the Renilla reniformis (Promega) reading and the TOP/FOP ratio was measured, as previously described [25 (link)]. Experiments were performed in triplicate.
Zheng A., Song X., Zhang L., Zhao L., Mao X., Wei M, & Jin F. (2019). Long non-coding RNA LUCAT1/miR-5582-3p/TCF7L2 axis regulates breast cancer stemness via Wnt/β-catenin pathway. Journal of Experimental & Clinical Cancer Research : CR, 38, 305.
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