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3 protocols using srcl2 6h2o

1

Synthesis and Characterization of PLLA Scaffolds

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PLLA with an inherent viscosity of 1.6 dL/g was purchased from Ji’nan Daigang (Ji Nan, China). d-Fructose (melting point [m.p.] 119°C–122°C) and Span 80 were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Dioxane, cyclohexane, and hexane were purchased from Sigma-Aldrich Co. Fetal bovine serum (FBS), minimum essential medium alpha medium (α-MEM), and trypsin were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Sodium dodecyl sulfate (SDS), dimethyl sulfoxide (DMSO), 3-[4,5-dimetylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT), glutaraldehyde, and bovine serum albumin (BSA) were obtained from Sigma-Aldrich Co. The assay kits of bicinchoninic acid (BCA) protein and alkaline phosphatase (ALP) were purchased from Beyotime Biotechnology (Northern Island, UK). The ActinGreen 488 and the 4′6-diamidino-2-phenylindole (DAPI) were purchased from Thermo Fisher Scientific. The mouse preosteoblast cell line (MC3T3-E1) was obtained from China Infrastructure of Cell Line Resource (Beijing, China). ICR mice and JW rabbits were purchased from Vital River Experimental Animal Co. Ltd. (Beijing, China). NaCl, KCl, K2HPO4·3H2O, CaCl2·2H2O, SrCl2·6H2O, MgCl2·6H2O, Na2SO4, and NaHCO3 were purchased from Sigma-Aldrich Co. and used without further purification.
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2

Synthesis of Photocatalytic Materials

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All the
chemicals were of analytical grade and were used as received without
further purification. In the syntheses, the following reagents were
involved: KCl (Sigma-Aldrich, ≥99.0%), NaCl (Merck, ≥99.7%),
TiO2 (P25, Degussa), Bi2O3 nanopowder
(Sigma Aldrich, 99.8%), HNO3 (VWR, 68%), SrCl2·6H2O (Sigma-Aldrich, ≥99.0%), NaOH (Fisher
Chemicals, ≥98.7%). Water used for the study was purified with
a system to produce ultrapure water (Purelab Option-Q7, ELGA). Commercially
available SrTiO3 nanopowder was used as a reference photocatalyst
(Sigma-Aldrich, 20–40 nm).
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3

Synthesis of Bi2O3-TiO2 Nanocomposites

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All chemicals were of analytical grade and were used as received without further purification. KCl (Sigma-Aldrich, ≥99.0%), NaCl (Merck, ≥99.7%), Bi2O3 nanopowder (Alfa Aesar, 99.8%), TiO2 (P25, Degussa), HNO3 (VWR, 68%), SrCl2·6H2O (Sigma-Aldrich, ≥99.0%), NaOH (Fisher Chemicals, ≥98.7%). The water used for the study was purified with a system to produce 18.2 MΩ cm ultra-pure water (Purelab Option-Q7, ELGA).
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