Pe conjugated anti mouse cd23 clone b3b4

B cells were purified from hCD40Tg/FcγR null mouse spleens by magnetic negative selection kit (StemCell Technologies, UK). Totally, 1 × 10⁵ B cells were incubated with various anti-CD40 mAbs at 10 µg/mL in 96-well round-bottom plates for 3 days and then analysed for surface expression of CD23 using PE-conjugated anti-mouse CD23 (clone B3B4, Biolegend) by flow cytometry.

Anti‐human ECP antibody (catalog no. E‐AB‐14971) was purchased from Elabscience; anti‐CD9 antibody (catalog no. ab92726) was purchased from Abcam. An ExoSparkler Exosome Membrane Labeling Kit (green) was purchased from Dojido Molecular Technologies. Alexa 647–conjugated donkey anti‐mouse IgG antibody was purchased from ThermoFisher. PerCP‐conjugated anti‐mouse CD3 (clone 145‐2C11), Pacific Blue–conjugated anti‐mouse CD4 (clone RM4‐5), phycoerythrin (PE)–conjugated anti‐mouse CXCR5 (clone 2G8), PerCP–Cy5.5–conjugated anti‐mouse B220 (clone RA3‐6B2), and PE‐conjugated anti‐mouse CD138 (clone 281‐2) antibodies were purchased from BD Biosciences. PE‐conjugated anti‐mouse CD44 (clone IM7), fluorescein isothiocyanate–conjugated anti‐mouse CD62L (clone MEL‐14), Pacific Blue–conjugated anti‐mouse CD21 (clone 7E9), and PE‐conjugated anti‐mouse CD23 (clone B3B4) antibodies were purchased from BioLegend. Alexa 488–conjugated anti‐mouse IL‐21 (bs‐2621R‐A488) antibody was purchased from Bioss. Enzyme‐linked immunosorbent assay (ELISA) kits for IL‐1β, IL‐6, and TNF were purchased from eBioscience. ELISA kits for antinuclear antibody (ANA), rheumatoid factor (RF), and anti–double‐stranded DNA (anti‐dsDNA) antibody were purchased from Alpha Diagnostic. The ELISA kit for human ECP was purchased from CUSABIO.