Pnr gal4

The following fly stocks were used in this study: w¹¹¹⁸ (BDSC#3601) as WT flies, y w hep¹ and y w hep^r75 [1 (link)], UAS-hep^act (BDSC#9306), puc^E69 (puc-Z)[54 (link)], 69B-GAL4 (BDSC#1774), pnr-GAL4 (BDSC#3039), en-GAL4 (gift from A. Brand), AS-GAL4 (c381-GAL4; BDSC#3734), en^X31 [55 (link)], UAS-en [56 (link)], UAS-HA::VP16::en [39 (link)], Scr¹⁷ (BDSC#3400), Antp^[Ns-rvC4] (BDSC#1830), Ubx¹ (BDSC#626), abdA^M1 and AbdB^D18 [57 (link)], UAS-Scr::HA, UAS-Ubx::HA and UAS-abdA::HA [58 (link)], UAS-Antp [59 (link)], UAS-AbdBm (BDSC#913), UAS-AbdBⁱ (VDRC#12024)[60 (link)], UAS-Dicer2 (BDSC#24650), hth^P2 [61 (link)], TM3,dfd-lacZ [62 (link)]. For live imaging, we used arm::GFP (BDSC#8556 on chromosome II; BDSC#8555 on chromosome III) and created the recombinant line arm::GFP,Abd-B^D18 that we balanced with TM3,twi-GAL4,UAS-GFP (BDSC#6663) to select the homozygote mutant embryos. We also created the following line for the Abd-B RNAi: UAS-Dicer2;UAS-CD8::RFP,UAS-Abd-Bⁱ, allowing the selection of AbdBⁱ embryos thanks to the expression of CD8::RFP.

Overexpression studies were performed using the Gal4/UAS system^{36 (link)}. We used the following UAS lines: UAS-Dl, UAS-Ser, UAS-mCD8-ChRFP (Bloomington Drosophila Stock Center; BDSC), UAS-fwdIR, UAS-sktlIR, UAS-plc-21IR, UAS-dgkIR, UAS-gfpIR (Vienna Drosophila RNAi Center). UAS-pld38, and UAS-rdgA/dgk were a generous gift from Padinjat Raghu (National Centre for Biological Sciences, Tata Institute of Fundamental Research, India). We also used the following Gal4 lines: pnr-Gal4, en-Gal4 and sca-Gal4 (BDSC). We also used the following strains Dl^[Rev10], Ser^[RX82], Spdo^G104, Ap-2α^[40–31], numb^{1537 (link)}. SpdoCh2GFP3, neur-nlsFP670, NiGFP were a generous gift from François Schweisguth (Institut Pasteur, France). UAS-lipinwt was a generous gift from Michael Lehmann (University of Arkansas, USA). All phenotypes were analysed at 25 °C unless stated otherwise.