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High sensitivity salivary cortisol enzyme immunoassay system

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The High sensitivity salivary cortisol enzyme immunoassay system is a laboratory equipment used for the quantitative determination of cortisol levels in human saliva samples. This system utilizes an enzyme-linked immunosorbent assay (ELISA) technique to measure the concentration of cortisol, a steroid hormone associated with the body's stress response.

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Lab products found in correlation

Powerwave ht microplate spectrophotometer, by Agilent Technologies (3 mentions) Precision series automated liquid handling system, by Agilent Technologies (3 mentions)

Spelling variants of this product

Enzyme immunoassay (22 citations) High-sensitivity enzyme immunoassay (3 citations) Cortisol (3 citations)

3 protocols using high sensitivity salivary cortisol enzyme immunoassay system

1

Salivary Cortisol Sampling and Analysis

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Unstimulated salivary samples were collected by passive drool in polypropylene vials and immediately iced. Samples were aliquoted into 1.8 nunc tubes and saliva was then frozen at −70°C until assayed. Samples were assayed in duplicate using a high sensitivity salivary cortisol enzyme immunoassay system that has an intra-assay precision (coefficient of variation, CV) of 3.35% – 3.65% with a sensitivity of <0.003 ug/dL (Salimetrics LLC). Samples were analyzed using a PowerWave HT Microplate Spectrophotometer in conjunction with a Precision Series Automated Liquid Handling System (BioTek Instruments, Inc.). Salivary samples were collected immediately before the drug cue paradigm and then at 15, 30 and 60 minutes post.
Back S.E., Gros D.F., McCauley J., Flanagan J., Cox E., Barth K, & Brady K.T. (2014). Laboratory-Induced Cue Reactivity among Individuals with Prescription Opioid Dependence. Addictive behaviors, 39(8), 1217-1223.
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2

Salivary Cortisol Collection and Analysis

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Unstimulated salivary samples were collected by passive drool in polypropylene vials and immediately iced. Samples were aliquoted into 1.8 nunc tubes and saliva was then frozen at-70 °C until ready for assay. Samples were assayed in duplicate using a high sensitivity salivary cortisol enzyme immunoassay system that has an intra-assay precision of 3.35%—3.65% with a lower sensitivity limit of <0.003 μg/dL (Salimetrics, LLC). Samples were analyzed using a PowerWave HT Microplate Spectrophotometer in conjunction with a Precision Series Automated Liquid Handling System (BioTek Instruments, Inc.).
Flanagan J.C., Allan N.P., Calhoun C.D., Badour C.L., Moran-Santa Maria M., Brady K.T, & Back S.E. (2018). Effects of Oxytocin on Stress Reactivity and Craving in Veterans with Co-Occurring PTSD and Alcohol Use Disorder. Experimental and clinical psychopharmacology, 27(1), 45-54.
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3

Salivary Cortisol Extraction and Analysis

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Unstimulated salivary samples were collected by passive drool in polypropylene vials and immediately iced. Samples were aliquoted into 1.8 nunc tubes and saliva was then frozen at −70 °C until assayed. Samples were assayed in duplicate using a high sensitivity salivary cortisol enzyme immunoassay system that has an intra-assay precision (coefficient of variation, CV) of 3.35%–3.65% with a lower sensitivity limit of <0.003 μg/dL (Salimetrics, LLC). Samples were analyzed using a PowerWave HT Microplate Spectrophotometer in conjunction with a Precision Series Automated Liquid Handling System (BioTek Instruments, Inc.).
Flanagan J.C., Fischer M.S., Nietert P.J., Back S.E., Maria M.M., Snead A, & Brady K.T. (2017). Effects of Oxytocin on Cortisol Reactivity and Conflict Resolution Behaviors among Couples with Substance Misuse. Psychiatry research, 260, 346-352.
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