A Shimadzu LC-16 (Shimazu, Kyoto, Japan) HPLC equipped with an SPD-16 UV-Vis detector and LC-16 pump was used in this work. A HPLC stainless-steel column (250 mm × 2.1 mm i.d.) and Alltech slurry packer (Alltech, Deerfield, IL, USA) were used for column packing. A Bruker DRX 500 spectrometer (Bruker, Bremen, Germany) was used to obtain the nuclear magnetic resonance (NMR) spectra. An SDT-650 thermal analyzer (TA Instruments, New Castle, DE, USA) was used to obtain the thermogravimetric analysis data. Mass spectrometry was carried out on a Bruker UltrafleXtreme MALDI-TOF mass spectrometer (Thermo Fisher, Bremen, Germany). Elemental analysis data were obtained on a Vario EL III elemental analyzer (Elementar, Langenselbold, Germany).
Ultraflextreme maldi tof mass spectrometer
Manufactured by Thermo Fisher Scientific
Sourced in Germany
The UltrafleXtreme MALDI-TOF mass spectrometer is a high-performance analytical instrument designed for the detection and identification of a wide range of molecules, including proteins, peptides, and small molecules. This instrument utilizes matrix-assisted laser desorption/ionization (MALDI) technology coupled with time-of-flight (TOF) mass analysis to provide accurate and reliable results.
Automatically generated - may contain errors
Lab products found in correlation
Vario el 3 elemental analyzer, by Elementar (1 mentions)
Lc 16, by Shimadzu (1 mentions)
Drx 500 spectrometer, by Bruker (1 mentions)
Yeast extract, by Research Products International (1 mentions)
Deoxynucleotides, by New England Biolabs (1 mentions)
Tryptone, by Research Products International (1 mentions)
C18 column, by Macherey-Nagel (1 mentions)
Orbitrap fusion esi ms, by Advion (1 mentions)
Triversa nanomate 100, by Advion (1 mentions)
S1000 thermal cycler, by Bio-Rad (1 mentions)
Restriction enzyme, by New England Biolabs (1 mentions)
T4 dna ligase, by New England Biolabs (1 mentions)
Q5 polymerase, by New England Biolabs (1 mentions)
Agilent 1200 series hplc, by Agilent Technologies (1 mentions)
2 protocols using ultraflextreme maldi tof mass spectrometer
1
Analytical Techniques for Material Characterization
2
Purification and Characterization of StsA
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Materials and reagents were purchased from
Gold Biotechnology, Fisher Scientific, or Sigma-Aldrich unless otherwise
noted. Yeast extract and tryptone were purchased from Research Products
International. Molecular biology reagents for cloning (e.g., restriction
enzymes, Q5 polymerase, T4 DNA ligase, and deoxynucleotides) were
purchased from New England Biolabs. Oligonucleotide primers and gene
blocks were obtained from Integrated DNA Technologies. DNA spin columns
were purchased from Epoch Life Sciences. Sanger sequencing was performed
by the Roy J. Carver Biotechnology Center (University of Illinois
at Urbana-Champaign). Polymerase chain reactions were performed using
a Bio-Rad S1000 thermal cycler. Escherichia coli DH5α and BL21(DE3) strains were used for plasmid maintenance
and protein overexpression, respectively. Expressed StsA was purified
using an Agilent 1200 series HPLC fitted with a 10 × 250 mm C18
column (Macherey Nagel). Mass spectroscopy was performed using a Bruker
Daltonics UltrafleXtreme MALDI-TOF mass spectrometer and a ThermoFisher
Scientific Orbitrap Fusion ESI-MS using an Advion TriVersa Nanomate
100.
Gold Biotechnology, Fisher Scientific, or Sigma-Aldrich unless otherwise
noted. Yeast extract and tryptone were purchased from Research Products
International. Molecular biology reagents for cloning (e.g., restriction
enzymes, Q5 polymerase, T4 DNA ligase, and deoxynucleotides) were
purchased from New England Biolabs. Oligonucleotide primers and gene
blocks were obtained from Integrated DNA Technologies. DNA spin columns
were purchased from Epoch Life Sciences. Sanger sequencing was performed
by the Roy J. Carver Biotechnology Center (University of Illinois
at Urbana-Champaign). Polymerase chain reactions were performed using
a Bio-Rad S1000 thermal cycler. Escherichia coli DH5α and BL21(DE3) strains were used for plasmid maintenance
and protein overexpression, respectively. Expressed StsA was purified
using an Agilent 1200 series HPLC fitted with a 10 × 250 mm C18
column (Macherey Nagel). Mass spectroscopy was performed using a Bruker
Daltonics UltrafleXtreme MALDI-TOF mass spectrometer and a ThermoFisher
Scientific Orbitrap Fusion ESI-MS using an Advion TriVersa Nanomate
100.
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