All native thin filament sliding assays were performed at room temperature (22 ± 1°C) using a Nikon Eclipse Ti-U inverted microscope equipped with a Plan Apo 100× 1.49 N.A. oil emersion lens for epifluorescence or through-the-objective total internal reflectance microscopy (TIRFM) using a custom “Micro Optic Fiber Launch TIRFM” (patent pending) as described (14 (link)). Briefly, either a Lumen 200-W metal arc lamp (Prior Scientific) or 532-nm diode-pumped, solid-state, 50-mW laser (Lasever Inc.) was used for excitation. Images were obtained using an intensified high-resolution 10-bit digital camera (XR/Turbo-Z running Piper Control v2.6.09 software, Stanford Photonics).
Plan apo 100 1.49 n a oil
Manufactured by Nikon
The Plan Apo 100× 1.49 N.A. oil objective is a high-magnification, high-numerical aperture objective lens designed for use in laboratory settings. It provides a 100× magnification and a numerical aperture of 1.49, which allows for high-resolution imaging and light gathering capabilities.
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2 protocols using plan apo 100 1.49 n a oil
1
Thin Filament Sliding Assay Protocol
2
Thin Filament Sliding Assay Protocol
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All native thin filament sliding assays were performed at room temperature (22 ± 1°C) using a Nikon Eclipse Ti-U inverted microscope equipped with a Plan Apo 100× 1.49 N.A. oil emersion lens for epifluorescence or through-the-objective total internal reflectance microscopy (TIRFM) using a custom “Micro Optic Fiber Launch TIRFM” (patent pending) as described (14 (link)). Briefly, either a Lumen 200-W metal arc lamp (Prior Scientific) or 532-nm diode-pumped, solid-state, 50-mW laser (Lasever Inc.) was used for excitation. Images were obtained using an intensified high-resolution 10-bit digital camera (XR/Turbo-Z running Piper Control v2.6.09 software, Stanford Photonics).
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