Nhbec

Two LSCC cell lines (AMC-HN-8 and HEP-2) and the normal human bronchial epithelial cell (NHBEC) line were purchased from Bena Culture Collection. All the cell lines were cultured in Dulbecco's Modified Eagle Medium (DMEM) containing 10% fetal bovine serum. Twenty pairs of primary LSCC paraneoplastic and carcinoma tissues were pooled from Beijing Tiantan Hospital, Affiliated to Capital Medical University. All patients were pathologically confirmed, and fresh tissue was collected from them immediately after surgery and frozen in liquid nitrogen. Tissue collection was approved by the ethical review committee at Beijing Tiantan Hospital, Affiliated to Capital Medical University.

Four LSCC cell lines (AMC-HN-8, HEP-2, TU-212 and TU-686) and the normal bronchial epithelial cell line NHBEC were purchased from Bena Culture Collection (Beijing, China). HEK293T cell line was purchased from American Type Culture Collection (ATCC) (Gaithersburg, MD, USA). All cell lines were cultured in DMEM medium (HyClone, Logan, Utah, USA) containing 10% fetal bovine serum (Biological Industries, Beit HaEmek, Israel) . Thirty pairs of primary LSCC paracancerous and cancerous tissues were collected from Chengde Central Hospital (Chengde, Hebei, China). All patients were pathologically confirmed, and the fresh tissues were immediately collected and frozen in liquid nitrogen after surgery. The collection of tissues was approved by the Review and Ethics committee of Chengde Central Hospital. Nuciferine (Purity, 99.49%) and other 49 natural products used in this study were all obtained from Selleck Chemicals (Houston, Texas, USA).