Ra16pa preamplifier

The collection of all electrophysiology recordings was done using a ZC16 headstage, RA16PA pre-amplifier, and RX5 Pentusa base station (Tucker-Davis Technologies, Alachua, FL). During data acquisition, the pre-amplifier high-pass filtered at 2.2 Hz, anti-alias filtered at 7.5 kHz, and sampled at a rate of 25 kHz. Recordings done with acute implants took place while the rats were under ketamine/xylazine anesthesia, while the chronic electrophysiology measurements were collected on awake and behaving animals, as described in Patel et al 2015. Each recording session lasted 3 and 10 min for acute and chronic implants, respectively. This study also analyzed chronic electrophysiology recordings from N = 6 animals reported in Patel et al 2016. The neural data was collected on N = 77 scissor cut PEDOT:pTS coated carbon fiber electrodes mounted on ZIF arrays.

Electrophysiology recordings using chronic implants of carbon fiber arrays and silicon probes were done while the rats were awake and moving about freely in their cage. All acquisition of electrophysiology recordings were taken using a ZC16 headstage, RA16PA preamplifier, and RX5 Pentusa base station (Tucker-Davis Technologies, Alachua, FL). During data acquisition, the pre-amplifier high pass filtered at 2.2 Hz, anti-aliased filtered at 7.5 kHz, and sampled at a rate of ∼25 kHz. Each recording session lasted 5 or 10 minutes.
Recording sessions were imported into Offline Sorter (Plexon, Dallas, TX) and first high-pass filtered (250 Hz corner, 4^th order Butterworth). Each channel was manually thresholded and the resultant waveforms sorted by a trained operator. Sorted waveforms belonging to the same neuronal unit were averaged together to obtain a peak-to-peak amplitude for that unit, which was averaged with all other unit peak-to-peak amplitude values to obtain the mean value for each recording day for each probe type. All reported values are mean ± standard error of the mean.