Poly(ε-caprolactone) (PCL, Mn = 80 kg/mol), poly(ε-caprolactone) diol (Mn = 530 g/mol), isophorone diisocyanate (IPDI), triphenylphosphine (TPP), phenothiazine (PTZ), dimethylterephthalate (DMT), Brij®35 and phosphate buffered saline (PBS) were purchased from Merck (Belgium). 2,6-di-tert-butyl-4-methylphenol (BHT) was obtained from Innochem (Belux). Deuterated Chloroform was purchased from EurIsotop (Belgium). Ethoxylated and propoxylated pentaerythritol triacrylate (EPPETA) and bismuth neodecanoate catalyst were kindly provided by Allnex (Belgium). Diphenyl(2,4,6-trimethylbenzoyl)phosphine oxide (TPO-L) was provided by Lambson (UK). Chloroform and acetone were purchased from ChemLab (Belgium). Hydrocortisone was purchased from the Apothecary of the Ghent University Hospital, (UZ Ghent, Ghent, Belgium). The skin samples were obtained from residual waste from a subcutaneous mastectomy. Small containers with 10% formol were purchased from VWR Chemicals (Fontenay-Sous-Bois, France) and automatic hematoxylin and eosin (HE) staining of the paraffin slices was used (T181 Tissue- Tek Prisma Plus, Sakura Finetek, Antwerp, Belgium).
T181 tissue tek prisma plus
Manufactured by Sakura Finetek
Sourced in Belgium
The T181 Tissue-Tek Prisma Plus is a fully automated staining instrument designed for routine histology and cytology applications. It provides efficient staining and coverslipping of tissue sections and smears, ensuring consistent and reliable results.
Automatically generated - may contain errors
Lab products found in correlation
Phenothiazine, by Merck Group (1 mentions)
Isophorone diisocyanate, by Merck Group (1 mentions)
Triphenylphosphine, by Merck Group (1 mentions)
Poly ε caprolactone, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Brij 35, by Merck Group (1 mentions)
Dimethyl terephthalate, by Merck Group (1 mentions)
Deuterated chloroform, by Eurisotop (1 mentions)
Acetone, by Chem-Lab (1 mentions)
Chloroform, by Chem-Lab (1 mentions)
Axio lab a1, by Zeiss (1 mentions)
2 protocols using t181 tissue tek prisma plus
1
Skin Tissue Engineering with PCL Biomaterials
2
Skin Penetration Capacity via CEDMN Patch
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Skin for ex vivo experiments was procured as residuary material after a patient had a subcutaneous mastectomy. Informed consent was obtained from the patient. The ex vivo skin sample was stretched and subsequently pierced several times with an 800 µm CEDMN patch.
The patch was then removed and the tissue was fixated in a 10% formol solution. Automatic
Haematoxylin and Eosin staining of the paraffin slices was used (T181 Tissue-Tek Prisma Plus, Sakura Finetek, Antwerp, Belgium). Skin penetration capacity was assessed by light microscopy (Axio Lab A1, Zeiss, Jena, Germany) of the 5 µm thick histological slices.
The patch was then removed and the tissue was fixated in a 10% formol solution. Automatic
Haematoxylin and Eosin staining of the paraffin slices was used (T181 Tissue-Tek Prisma Plus, Sakura Finetek, Antwerp, Belgium). Skin penetration capacity was assessed by light microscopy (Axio Lab A1, Zeiss, Jena, Germany) of the 5 µm thick histological slices.
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