Ptprm

Five-micrometer sections of paraffin-embedded human EOCs, borderline tumors, benign tumors, and normal ovarian tissues were prepared for staining. After dewaxing, the sections were rehydrated, then antigen retrieval and endogenous peroxidase blocking were performed. The slides were incubated with monoclonal antibodies (PTPRM: Santa Cruz, 1:200) overnight at 4 °C. The sections were then incubated with biotinylated anti-mouse secondary antibody (1:100) followed by horseradish peroxidase-streptavidin. Antigens were detected with peroxidase substrate and counterstained with hematoxylin.The primary antibody was replaced with phosphate buffered saline(PBS) as the negative control.Immunohistochemical staining of all sections was performed under the same conditions and at the same staining time. PTPRM was localized in the cytoplasm and/or nucleus, and cells with brownish-yellow coloration in the cytoplasm and/or nucleus were considered positive cells.Each specimen was randomly selected from 10 fields of view under a 400 × light microscope, and the number of positive cells in the 100 cells of the fields was counted(If the total number of cells in one field was less than 100, then 100 cells were counted in the adjacent two fields). The average number of positive cells was taken as the positive percentage, and if the number was greater than 30%, it was considered positive.