Diaminobenzidine tetrahydroxychloride solution

Manufactured by Vector Laboratories

Sourced in United States

Diaminobenzidine tetrahydroxychloride solution is a chromogenic substrate used in immunohistochemistry and enzyme-linked immunosorbent assays (ELISA) for the detection of specific antigens. It produces a brown color reaction in the presence of peroxidase, a commonly used enzyme label in these techniques.

Automatically generated - may contain errors

Lab products found in correlation

Gtx125918, by GeneTex (1 mentions) Abc kit, by Vector Laboratories (1 mentions) Gtx113617, by GeneTex (1 mentions) Antigen unmasking solution, by Vector Laboratories (1 mentions) Pab1620, by Merck Group (1 mentions)

2 protocols using diaminobenzidine tetrahydroxychloride solution

Immunohistochemical Analysis of Snail and GFP

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue sections were dewaxed and rehydrated. Antigen retrieval was performed by incubating the slides in 10 mmol/L sodium citrate buffer and microwaving the samples for 20 minutes. After blocking with 3% H₂O₂ and 10% normal goat serum (NGS), the slides were incubated with rabbit polyclonal antibodies against Snail (1:200; GTX125918; GeneTex, Inc., San Antonio, TX, USA) or GFP (1:500; GTX 113617; GeneTex) at 4°C overnight. The slides were then incubated with a biotin‐conjugated anti‐rabbit secondary antibody followed by polymer‐HRP reagent, using an ABC kit from Vector Laboratories (Burlingame, CA, USA). Peroxidase activity was visualized with a diaminobenzidine tetrahydroxychloride solution (Vector Laboratories). The sections were counterstained with hematoxylin.

Liu Y., Chen C., Yen C., Tung C., Chen C., Chen Y.A, & Huang M. (2018). Human immunodeficiency virus Tat‐TIP30 interaction promotes metastasis by enhancing the nuclear translocation of Snail in lung cancer cell lines. Cancer Science, 109(10), 3105-3114.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Breast Cancer Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Breast cancer tissue arrays containing 150 paraffin-embedded sections of normal and malignant tissues with pathological information (stages I through III) were obtained from US Biomax, Inc (BR1503b). Slides were deparaffinized, hydrated, and treated with antigen unmasking solutions (Vector Laboratories, Inc.). After being blocked with 0.3% H₂O₂ and nonimmune goat serum, sections were incubated at room temperature with an antibody against S-nitrosocysteine (Abcam, Cambridge, MA, USA, clone HY8E12), human LAMA3 (R&D Systems, Minneapolis, MN, USA, , clone 546215) or wild-type human p53 (EMD Millipore, clone pAb1620) and link antibodies, followed by peroxidase-conjugated streptavidin complex and diaminobenzidine tetrahydroxy chloride solution as the peroxidase substrate (Vector Laboratories, Burlingame, CA, USA). The sections were counterstained with hematoxylin. Photomicrographs were taken with the Zeiss Axioskop Imaging platform and Axion Vision software (Version 4.7).

Furuta S., Ren G., Mao J.H, & Bissell M.J. (2018). Laminin signals initiate the reciprocal loop that informs breast-specific gene expression and homeostasis by activating NO, p53 and microRNAs. eLife, 7, e26148.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!