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Anti human mouse myeloperoxidase mpo

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Anti-human/mouse myeloperoxidase (MPO) is a laboratory reagent used to detect and quantify the presence of myeloperoxidase, an enzyme found in the granules of neutrophils and monocytes. This antibody can be used for immunohistochemistry, immunocytochemistry, and ELISA applications.

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Lab products found in correlation

4 6 diamidino 2 phenylindole dihydrochloride dapi, by Thermo Fisher Scientific (2 mentions) Anti p47, by Santa Cruz Biotechnology (2 mentions) Anti neutrophil elastase, by Abcam (2 mentions) Anti c albicans, by Merck Group (2 mentions) Anti p40, by Merck Group (2 mentions) Anti cd63, by Merck Group (2 mentions) Anti histone h3 citrulline r2 r8 r17, by Abcam (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Abcam (2 mentions)

Close spelling variants of this product

Anti-Myeloperoxidase/MPO Myeloperoxidase (MPO) Human MPO Mouse MPO Anti-MPO

2 protocols using anti human mouse myeloperoxidase mpo

1

Neutrophil Immunofluorescence Staining

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5×104 human peripheral neutrophils were plated on glass coverslips 1 h prior to stimulation. After stimulation, cells were fixed with 2% paraformaldehyde for 20 min at 37°C. Immunofluorescence staining was performed as described elsewhere. Anti-neutrophil elastase (NE) (Abcam), 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (Life technologies), anti-human/mouse myeloperoxidase (MPO) (R&D Systems), anti-C. albicans (Acris), anti-p40 (Millipore, 1.22), anti-p47 (Santa Cruz), anti-CD63 (Millipore, RFAC4). For lung histology dissected lungs of infected mice were fixed over night in 2% paraformaldehyde and embedded in wax for sectioning. Sections were treated with a standard antigen retrieval protocol and immunofluorescence staining as described elsewhere. Cit-H3: anti-Histone H3 (citrulline R2 + R8 + R17) (Abcam, ab5103), MPO, DAPI. Stained cells and tissues were mounted and examined with confocal microscopy. Image analysis was performed using ImageJ.
Branzk N., Lubojemska A., Hardison S.E., Wang Q., Gutierrez M.G., Brown G.D, & Papayannopoulos V. (2014). Neutrophils sense microbial size and selectively release neutrophil extracellular traps in response to large pathogens. Nature immunology, 15(11), 1017-1025.
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2

Neutrophil Immunofluorescence Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
5×104 human peripheral neutrophils were plated on glass coverslips 1 h prior to stimulation. After stimulation, cells were fixed with 2% paraformaldehyde for 20 min at 37°C. Immunofluorescence staining was performed as described elsewhere. Anti-neutrophil elastase (NE) (Abcam), 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (Life technologies), anti-human/mouse myeloperoxidase (MPO) (R&D Systems), anti-C. albicans (Acris), anti-p40 (Millipore, 1.22), anti-p47 (Santa Cruz), anti-CD63 (Millipore, RFAC4). For lung histology dissected lungs of infected mice were fixed over night in 2% paraformaldehyde and embedded in wax for sectioning. Sections were treated with a standard antigen retrieval protocol and immunofluorescence staining as described elsewhere. Cit-H3: anti-Histone H3 (citrulline R2 + R8 + R17) (Abcam, ab5103), MPO, DAPI. Stained cells and tissues were mounted and examined with confocal microscopy. Image analysis was performed using ImageJ.
Branzk N., Lubojemska A., Hardison S.E., Wang Q., Gutierrez M.G., Brown G.D, & Papayannopoulos V. (2014). Neutrophils sense microbial size and selectively release neutrophil extracellular traps in response to large pathogens. Nature immunology, 15(11), 1017-1025.
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