Commasie plus protein assay reagent

Fetal bovine serum (FBS), Roswell Park Memorial Institute (RPMI)-1640 and penicillin-streptomycin were purchased from Gibco BRL Company (Grand Island, NY, USA). Modified Radioimmunoprecipitation assay (RIPA) lysis buffer, protease inhibitor cocktail, Commasie Plus™ Protein Assay Reagent, and chemiluminescent immunoblotting reagent were obtained from Thermo Fisher Scientific (Rockford, IL, USA). Syringic acid, pyrogallol, p-coumaric acid, catechin, gallic acid, protocatechuic acid, chlorogenic acid, caffeic acid, vanillic acid, ferulic acid, and ellagic acid, Sulforhodamine B (SRB) reagent, mitochondria membrane potential kit (JC-10 dye), propidium iodide (PI) and anti-β-actin antibody were obtained from Sigma-Aldrich (St. Louis, MO, USA). Primary antibody of PARP-1 was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Primary antibodies of cyclin D1, cyclin E, CDK-4, CDK-6, cleaved-caspase3, phospho-GSK-3β, GSK-3β (Ser9), phospho-β-catenin, β-catenin, phospho-Akt, Akt, and horseradish peroxidase-conjugated anti-mouse or rabbit-IgG were purchased from Cell Signaling Technology (Beverly, MA, USA). Apoptosis assay kits were obtained from Bio-Legend (San Diego, CA, USA).