Dmem with 4.5 g l d glucose

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom

DMEM with 4.5 g/L d-glucose is a cell culture medium designed to support the growth and maintenance of a wide variety of cell types. It contains 4.5 grams of d-glucose per liter as a source of energy for cells.

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Lab products found in correlation

L glutamine solution, by Merck Group (2 mentions) Penicillin streptomycin, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Mem non essential amino acid solution 100, by Merck Group (1 mentions) Mem non essential amino acid solution 100x, by Merck Group (1 mentions) Celltiter 96 aqueous one solution cell proliferation assay mts, by Promega (1 mentions)

Close spelling variants of this product

Knockout DMEM with 4.5 g/ L d-glucose DMEM with 4.5 g/L glucose DMEM (4.5 g/L d-glucose; 4.5 g/L D-glucose 4.5 g/L glucose D-glucose DMEM DMEM-glucose D-glucose L-glucose L-DMEM

2 protocols using dmem with 4.5 g l d glucose

Murine Fibroblast Cell Culture on Nanofiber Membranes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Murine
fibroblast (NIH
3T3) cells were used for cell culture. Cells were seeded on nano-
and microfiber PVB membranes sterilized with UV light at concentration
2 × 10⁴ cells per sample. Cell culture was carried
out in an incubator at 37 °C and an atmosphere with a concentration
of 5% CO₂ and humidity about 90%. NIH 3T3 cells were cultured
in a complete culture medium composed of Dulbecco’s modified
Eagle medium (DMEM with 4.5 g/L d-glucose, Gibco, UK), supplemented
with 10% of fetal bovine serum (FBS, Sigma-Aldrich, USA), 2% of antibiotics
(penicillin–streptomycin, Sigma-Aldrich, USA), 1% of l-glutamine solution (Sigma-Aldrich, USA), and 1% of aminoacids (Mem
nonessential amino acid solution 100×, Sigma-Aldrich, USA). The
medium was changed three times a week, except samples for lactate
dehydrogenase (LDH) assay.

Krysiak Z.J., Kaniuk Ł., Metwally S., Szewczyk P.K., Sroczyk E.A., Peer P., Lisiecka-Graca P., Bailey R.J., Bilotti E, & Stachewicz U. (2020). Nano- and Microfiber PVB Patches as Natural Oil Carriers for Atopic Skin Treatment. ACS Applied Bio Materials, 3(11), 7666-7676.

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NIH 3T3 Fibroblast Cell Proliferation and Adhesion

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NIH 3T3 murine fibroblast cells (Sigma Aldrich, Gillingham, UK) were used for proliferation and adhesion assay on the meshes sterilized with UV light. Cells were seeded on PS, PA6, and PS-PA6 composite fibers, and on the bottom of a 24-well plate (TCPS) as a reference, with a concentration of 2 × 10⁴ cells per sample. Samples with cells were incubated at H = 90%, T = 37 °C, and CO₂ set to 5% up to 7 days. Cell proliferation and adhesion assays were performed using MTS (CellTiter 96^®® AQueous One Solution Cell Proliferation Assay, MTS, Promega, Madison, WI, USA). The cell morphology was examined with SEM. NIH 3T3 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM with 4.5 g/L D-Glucose, Gibco, Paisley, UK), supplemented with 10% of Fetal Bovine Serum (FBS, Sigma Aldrich, St. Louis, MO, USA), 1% of L-Glutamine solution (Sigma Aldrich, St. Louis, MO, USA), 2% of antibiotics (Penicillin-Streptomycin, Sigma Aldrich, St. Louis, MO, USA), and 1% of amino acids (Mem non-essential Amino Acid solution 100x, Sigma Aldrich, St. Louis, MO, USA). The medium was changed three times a week.

Krysiak Z.J., Gawlik M.Z., Knapczyk-Korczak J., Kaniuk Ł, & Stachewicz U. (2020). Hierarchical Composite Meshes of Electrospun PS Microfibers with PA6 Nanofibers for Regenerative Medicine. Materials, 13(8), 1974.

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