We collected DNA using sterile cotton buccal swabs (Sarstedt AG, Germany) and extracted it by applying the QIAamp DNA Mini kit (Qiagen, Germany). Repeat length polymorphisms (AR(CAG), AR(GGN), DAT1(VNTR), ERα(TA) and ERβ(CA)) were investigated by PCR with fluorescent-dye-labeled primers and capillary electrophoresis. The single base primer extension (SBE) method also known as minisequencing was applied for the typing of single nucleotide polymorphism (SNP) variants (Val158Met) in the COMT gene.
Sterile cotton buccal swabs
Manufactured by Sarstedt
Sourced in Germany
Sterile cotton buccal swabs are single-use sampling devices designed for the collection of cells or fluids from the inner cheek or oral cavity. They feature a cotton-tipped applicator attached to a plastic handle, ensuring a secure grip during the sampling process. These swabs are packaged in a sterile manner to maintain sample integrity.
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Lab products found in correlation
3 protocols using sterile cotton buccal swabs
1
DNA Profiling: Genetic Markers Analysis
2
DNA Profiling: Genetic Markers Analysis
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We collected DNA using sterile cotton buccal swabs (Sarstedt AG, Germany) and extracted it by applying the QIAamp DNA Mini kit (Qiagen, Germany). Repeat length polymorphisms (AR(CAG), AR(GGN), DAT1(VNTR), ERα(TA) and ERβ(CA)) were investigated by PCR with fluorescent-dye-labeled primers and capillary electrophoresis. The single base primer extension (SBE) method also known as minisequencing was applied for the typing of single nucleotide polymorphism (SNP) variants (Val158Met) in the COMT gene.
3
Genetic Profiles in Testosterone Study
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DNA was collected using sterile cotton buccal swabs (Sarstedt AG, Germany), and was extracted applying the QIAamp DNA Mini kit (Qiagen, Germany). AR(CAG)-repeat and DAT1 VNTR polymorphisms were investigated by PCR with fluorescent-labeled primers and capillary electrophoresis (details of procedure in SI). Treatment groups (Testosterone M = 22.18, SD = 3.11, Placebo M = 22.33, SD = 2.83) did not differ in mean AR(CAG) repeat length (z = 0.34, p = .736). The 9/10R and the 10/10R genotypes accounted for most of the observed DAT1 genotypes in our sample (33% (n=56) and 57% (n=96), respectively, see SI), and we thus used these two genotypes in the analyses.
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