Chamq sybr qpcr master mix low rox premixed kit

Primer 5'-3'
The ChamQ TM SYBR ® qPCR Master Mix (Low ROX Premixed) kit and 7500 real-time instrument (Applied Biosystems, Foster City, CA, USA) were used for qRT-PCR. The kit instructions were followed, and the reaction mixture was prepared on ice.

To understand the phosphate-solubilization mechanism of the WS-FJ9 strain from multiple angles, the phosphate solubilization genes from different phosphate solubilization pathways were selected to detect their relative expression levels by qRT-PCR, including the PhoR gene responsible for sensing the twocomponent system of external phosphate sources, which can sense the concentration of soluble phosphate in the outside world; phosphatase genes AP-1, AP-2, AP-3, which encode the acid phosphatase gene and are important enzymes regulating phosphorus metabolism; organic acid genes, such as PhoA, which encode alkaline phosphatase, which can mineralize the activity of organic acids; and HlyB, GspE, and GspF, which are related to the secretion system responsible for secreting organic acids and enzymes into the environment. Primer 5.0 software was used to design speci c primers for quantitative real-time PCR. The speci c primers were designed as follows:
Table 1. Specific primers for qRT-PCR of Burkholderia multivorans WS-FJ9 phosphate-solubilizing genes Gene Name Primer (5'-3')
The ChamQ TM SYBR ® qPCR Master Mix (Low ROX Premixed) kit and a 7500 real-time instrument (Applied Biosystems, Foster City, CA, USA) were used for qRT-PCR. The kit instructions were followed, and the reaction mixture was prepared on ice.