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Drg hepcidin 25 bioactive high sensitive elisa kit

Manufactured by DRG International

The DRG Hepcidin 25 (bioactive) High Sensitive ELISA kit is a quantitative in vitro diagnostic test used for the measurement of Hepcidin-25 levels in human serum and plasma samples. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) principle to detect and quantify the target analyte.

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6 protocols using drg hepcidin 25 bioactive high sensitive elisa kit

1

Birth Cohort Study on Vaccination Response

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EMaBS is a prospective birth cohort that was originally designed as an RCT to test whether anthelminthic treatment during pregnancy and early childhood was associated with differential response to vaccination or incidence of infections, such as pneumonia, diarrhea or malaria (http://emabs.lshtm.ac.uk/)45 (link). This cohort was part of the VaccGene study as described elsewhere46 . Blood samples were collected in Vacutainer tubes containing EDTA at birth and at subsequent birthdays up to 5 years of age. Anthropometry and biomarkers of iron and inflammation were measured in samples from a single annual visit based on the availability of stored samples. Serum ferritin (Chemiluminescent Microparticle Immunoassay, Abbott Architect), CRP (Multigent CRP Vario assay, Abbott Architect), hepcidin (DRG Hepcidin-25 (bioactive) High Sensitive ELISA kit (DRG International)), hemoglobin (Coulter analyzer, Beckman Coulter) and malaria parasitemia (Giemsa-stained thick and thin blood films) were measured. Genotyping of sickle cell trait in the VaccGene study is described below.
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2

Longitudinal Malaria Immunity Evaluation

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This was an ongoing rolling longitudinal study designed to evaluate immunity to malaria in children and is described elsewhere40 (link). Within this cohort, children were followed to 8 years of age with weekly follow-ups and annual cross-sectional surveys during which anthropometry measurements were made and blood samples were collected. Serum ferritin (Chemiluminescent Microparticle Immunoassay, Abbott Architect), hepcidin (DRG Hepcidin 25 (bioactive) High Sensitive ELISA kit (DRG International)), CRP (Multigent CRP Vario assay, Abbott Architect), hemoglobin (Coulter analyzer, Beckman Coulter) and malaria parasitemia (Giemsa-stained thick and thin blood films) were measured from blood samples collected at a single cross-sectional survey based on the availability of plasma samples archived at 80°C. Genotyping of hemoglobin types and α-thalassemia was conducted by PCR35 (link),36 (link) using DNA extracted with the Qiagen DNA Blood Mini kit (Qiagen).
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3

Birth Cohort Study on Vaccination Response

Check if the same lab product or an alternative is used in the 5 most similar protocols
EMaBS is a prospective birth cohort that was originally designed as an RCT to test whether anthelminthic treatment during pregnancy and early childhood was associated with differential response to vaccination or incidence of infections, such as pneumonia, diarrhea or malaria (http://emabs.lshtm.ac.uk/)45 (link). This cohort was part of the VaccGene study as described elsewhere46 . Blood samples were collected in Vacutainer tubes containing EDTA at birth and at subsequent birthdays up to 5 years of age. Anthropometry and biomarkers of iron and inflammation were measured in samples from a single annual visit based on the availability of stored samples. Serum ferritin (Chemiluminescent Microparticle Immunoassay, Abbott Architect), CRP (Multigent CRP Vario assay, Abbott Architect), hepcidin (DRG Hepcidin-25 (bioactive) High Sensitive ELISA kit (DRG International)), hemoglobin (Coulter analyzer, Beckman Coulter) and malaria parasitemia (Giemsa-stained thick and thin blood films) were measured. Genotyping of sickle cell trait in the VaccGene study is described below.
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4

Malaria vaccine trial in Burkina Faso

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This was part of the VaccGene study, which aimed to identify genetic variants associated with differential response to vaccination in infancy but with ethical approval to undertake analyses to examine the effect of iron status on infection susceptibility. Details of the study design are described elsewhere37 (link). Infants between the ages of 6 and 18 months living in the Banfora region of Burkina Faso were recruited into a phase 1–2b clinical trial to test the safety, immunogenicity and efficacy of an experimental heterologous viral-vectored prime-boost liver-stage malaria vaccine37 (link). Serum ferritin (Chemiluminescent Microparticle Immunoassay, Abbott Architect), hepcidin (DRG Hepcidin 25 (bioactive) High Sensitive ELISA kit (DRG International)), CRP (Multigent CRP Vario assay, Abbott Architect), hemoglobin (Coulter analyzer, Beckman Coulter) and malaria parasitemia (Giemsa-stained thick and thin blood films) were measured at a single time point. Genotyping of sickle cell trait in the VaccGene study is described below.
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5

Malaria vaccine trial in Burkina Faso

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This was part of the VaccGene study, which aimed to identify genetic variants associated with differential response to vaccination in infancy but with ethical approval to undertake analyses to examine the effect of iron status on infection susceptibility. Details of the study design are described elsewhere37 (link). Infants between the ages of 6 and 18 months living in the Banfora region of Burkina Faso were recruited into a phase 1–2b clinical trial to test the safety, immunogenicity and efficacy of an experimental heterologous viral-vectored prime-boost liver-stage malaria vaccine37 (link). Serum ferritin (Chemiluminescent Microparticle Immunoassay, Abbott Architect), hepcidin (DRG Hepcidin 25 (bioactive) High Sensitive ELISA kit (DRG International)), CRP (Multigent CRP Vario assay, Abbott Architect), hemoglobin (Coulter analyzer, Beckman Coulter) and malaria parasitemia (Giemsa-stained thick and thin blood films) were measured at a single time point. Genotyping of sickle cell trait in the VaccGene study is described below.
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6

Longitudinal Malaria Immunity Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols
This was an ongoing rolling longitudinal study designed to evaluate immunity to malaria in children and is described elsewhere40 (link). Within this cohort, children were followed to 8 years of age with weekly follow-ups and annual cross-sectional surveys during which anthropometry measurements were made and blood samples were collected. Serum ferritin (Chemiluminescent Microparticle Immunoassay, Abbott Architect), hepcidin (DRG Hepcidin 25 (bioactive) High Sensitive ELISA kit (DRG International)), CRP (Multigent CRP Vario assay, Abbott Architect), hemoglobin (Coulter analyzer, Beckman Coulter) and malaria parasitemia (Giemsa-stained thick and thin blood films) were measured from blood samples collected at a single cross-sectional survey based on the availability of plasma samples archived at 80°C. Genotyping of hemoglobin types and α-thalassemia was conducted by PCR35 (link),36 (link) using DNA extracted with the Qiagen DNA Blood Mini kit (Qiagen).
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