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Cellometer automated cell counter

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The Cellometer Automated Cell Counter is a laboratory instrument designed to accurately count and analyze cells. It utilizes automated digital imaging and advanced algorithms to provide rapid and reliable cell counts across a variety of cell types.

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Lab products found in correlation

Qubit 2.0 fluorometer, by Thermo Fisher Scientific (4 mentions) Single cell 3 rnaseq technology, by 10x Genomics (2 mentions) Agilent high sensitive dna kit, by Agilent Technologies (2 mentions) Nextseq 2000, by Illumina (2 mentions) Tryple, by Thermo Fisher Scientific (2 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (2 mentions)

Close spelling variants of this product

Cellometer Mini Automated Cell Counter Cellometer Mini Brightfield Automated Cell Counter Cellometer cell counter Automated cell counter Cell counter Cellometer

4 protocols using cellometer automated cell counter

1

Single-cell RNA-seq of DHT-treated human islets

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Human islets (750 IEQ per condition) were treated with either 10nM DHT or EtOH overnight in a humidified incubator containing 4% CO2 at 37°C. Islet cells were then dispersed using TrypLE (Thermofischer), and immediately evaluated for viability (89.88 ± 1.77%) by Cellometer Automated Cell Counter (Nexcelom Bioscience) prior to single cell RNAseq library preparation. For 10x single cell RNAseq library preparation, 5000 individual live cells per sample were targeted by using 10x Single Cell 3′ RNAseq technology provided by 10x Genomics (10X Genomics Inc). Briefly, viable single cell suspensions were partitioned into nanoliter-scale Gel Beads-In-EMulsion (GEMs). Full-length barcoded cDNAs were then generated and amplified by PCR to obtain sufficient mass for library construction. Following enzymatic fragmentation, end-repair, A-tailing, and adaptor ligation, single cell 3′ libraries comprising standard Illumina P5 and P7 paired-end constructs were generated. Library quality controls were performed by using Agilent High Sensitive DNA kit with Agilent 2100 Bioanalyzer (Agilent) and quantified by Qubit 2.0 fluorometer (ThermoFisher). Pooled libraries at a final concentration of 750pM were sequenced with paired end single index configuration by Illumina NextSeq 2000 (Illumina).
Xu W., Qadir M.M., Nasteska D., de Sa P.M., Gorvin C.M., Blandino-Rosano M., Evans C.R., Ho T., Potapenko E., Veluthakal R., Ashford F.B., Bitsi S., Fan J., Bhondeley M., Song K., Sure V.N., Sakamuri S.S., Schiffer L., Beatty W., Wyatt R., Frigo D.E., Liu X., Katakam P.V., Arlt W., Buck J., Levin L.R., Hu T., Kolls J., Burant C.F., Tomas A., Merrins M.J., Thurmond D.C., Bernal-Mizrachi E., Hodson D.J, & Mauvais-Jarvis F. (2023). Architecture of androgen receptor pathways amplifying glucagon-like peptide-1 insulinotropic action in male pancreatic β cells. Cell reports, 42(5), 112529.
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2

Cell Proliferation Assay for H69, NHC, and PSC

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Cell proliferation assays were done on H69, NHC, and PSC cells by performing cell counts. Briefly, all cells were plated at a concentration of 100,000 cells/well (day 0) in 6-well plates. Cells were trypsinized, resuspended in media, and counted on days 1–5 post-plating using a Cellometer Automated Cell Counter (Nexcelom Bioscience). Cell population doubling time was calculated using the formula: ln(# cells harvested)−ln(# cells seeded)/ln2.
Tabibian J.H., Trussoni C.E., O’Hara S.P., Splinter P.L., Heimbach J.K, & LaRusso N.F. (2014). Characterization of cultured cholangiocytes isolated from livers of patients with primary sclerosing cholangitis. Laboratory investigation; a journal of technical methods and pathology, 94(10), 1126-1133.
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3

Single-cell RNA-seq of DHT-treated human islets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human islets (750 IEQ per condition) were treated with either 10nM DHT or EtOH overnight in a humidified incubator containing 4% CO2 at 37°C. Islet cells were then dispersed using TrypLE (Thermofischer), and immediately evaluated for viability (89.88 ± 1.77%) by Cellometer Automated Cell Counter (Nexcelom Bioscience) prior to single cell RNAseq library preparation. For 10x single cell RNAseq library preparation, 5000 individual live cells per sample were targeted by using 10x Single Cell 3′ RNAseq technology provided by 10x Genomics (10X Genomics Inc). Briefly, viable single cell suspensions were partitioned into nanoliter-scale Gel Beads-In-EMulsion (GEMs). Full-length barcoded cDNAs were then generated and amplified by PCR to obtain sufficient mass for library construction. Following enzymatic fragmentation, end-repair, A-tailing, and adaptor ligation, single cell 3′ libraries comprising standard Illumina P5 and P7 paired-end constructs were generated. Library quality controls were performed by using Agilent High Sensitive DNA kit with Agilent 2100 Bioanalyzer (Agilent) and quantified by Qubit 2.0 fluorometer (ThermoFisher). Pooled libraries at a final concentration of 750pM were sequenced with paired end single index configuration by Illumina NextSeq 2000 (Illumina).
Xu W., Qadir M.M., Nasteska D., de Sa P.M., Gorvin C.M., Blandino-Rosano M., Evans C.R., Ho T., Potapenko E., Veluthakal R., Ashford F.B., Bitsi S., Fan J., Bhondeley M., Song K., Sure V.N., Sakamuri S.S., Schiffer L., Beatty W., Wyatt R., Frigo D.E., Liu X., Katakam P.V., Arlt W., Buck J., Levin L.R., Hu T., Kolls J., Burant C.F., Tomas A., Merrins M.J., Thurmond D.C., Bernal-Mizrachi E., Hodson D.J, & Mauvais-Jarvis F. (2023). Architecture of androgen receptor pathways amplifying glucagon-like peptide-1 insulinotropic action in male pancreatic β cells. Cell reports, 42(5), 112529.
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4

Cell Proliferation Assay for H69, NHC, and PSC

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell proliferation assays were done on H69, NHC, and PSC cells by performing cell counts. Briefly, all cells were plated at a concentration of 100,000 cells/well (day 0) in 6-well plates. Cells were trypsinized, resuspended in media, and counted on days 1–5 post-plating using a Cellometer Automated Cell Counter (Nexcelom Bioscience). Cell population doubling time was calculated using the formula: ln(# cells harvested)−ln(# cells seeded)/ln2.
Tabibian J.H., Trussoni C.E., O’Hara S.P., Splinter P.L., Heimbach J.K, & LaRusso N.F. (2014). Characterization of cultured cholangiocytes isolated from livers of patients with primary sclerosing cholangitis. Laboratory investigation; a journal of technical methods and pathology, 94(10), 1126-1133.
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