Goat anti rabbit igg hrp

Male C57BL/6 mice with average weights of 23–26 g were purchased from the Corporation of Lingchang Biological Technology (Shanghai, China). The animals were allowed to acclimate to their surroundings for 1 week. LPS was purchased from Sigma (USA). LFM-A13 was purchased from Topscience (Shanghai, China). Kits for protein extraction, BCA protein assay, SDS-PAGE gel preparation, Western blot analysis, hematoxylin and eosin (H&E staining), TUNEL apoptosis assay, mouse IL-4 enzyme-linked immunosorbent assay (ELISA), mouse IL-6 ELISA, mouse TNF-α ELISA, and myeloperoxidase (MPO) colorimetric activity assay were purchased from KeyGEN (Nanjing, China). Neutral gum, methanol, ethanol, and xylene were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Triton-X100 was purchased from KeyGEN (Nanjing, China). Rabbit anti-GAPDH (10B8) and goat antirabbit IgG-HRP were purchased from KeyGEN (Nanjing, China). Rabbit anti-p-BTK (ab52192) was purchased from Abcam (Shanghai, China). Rabbit anti-BTK (DF6472) was purchased from Affinity Biosciences (Cincinnati, USA).

ACHN cells, a renal cell carcinoma cell line, were obtained from the Cell Bank of the Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College (Beijing, China). The Trizol™ reagent was obtained from Thermo Fisher Scientific (Waltham, MA, USA). Rabbit anti-HMGA2 antibody (Ab207301), Rabbit anti-E- cadherin antibody (Ab40772), Rabbit anti-N-cadherin antibody (Ab76011), Rabbit anti-Snail antibody (Ab216347) were purchased from Abcam (Boston, MA, USA). Goat anti-rabbit IgG-HRP was purchased from KeyGenBioTECH (Nanjing, China). The Reverse Transcription kit and One Step TB Green™ PrimeScript™ RT-PCR Kit II (SYBR Green) were purchased from TaKaRa-Bio (Dalian, China). The primers were ordered from Beijing Aoke Biotechnology Co., Ltd. (Beijing, China). The bicinchoninic acid (BCA) protein assay kit was purchased from Shenyang Wanlei Biological Technology Co. Ltd. (Shenyang, China). Three HMGA2 shRNAs and negative control scrambled shRNA were custom-designed and synthesized by KeyGen BioTECH (Nanjing, China).