Sybr green qpcr master mix

Manufactured by ExCell Bio

Sourced in China

SYBR Green qPCR Master Mix is a ready-to-use solution for quantitative real-time PCR (qPCR) assays. It contains the necessary components, including SYBR Green I dye, DNA polymerase, and buffer, to amplify and detect target DNA sequences.

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Lab products found in correlation

Topotecan, by Merck Group (5 mentions) Paclitaxel, by Merck Group (5 mentions) Fumitremorgin c ftc, by Merck Group (4 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions) P erk sc 7383, by Santa Cruz Biotechnology (3 mentions) Cisplatin, by Merck Group (3 mentions) Verapamil, by Merck Group (3 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Hoechst 33342, by Merck Group (2 mentions) Rho123, by Merck Group (2 mentions) Flow cytometry antibodies against abcb1 557002, by BD (2 mentions) P akt, by Santa Cruz Biotechnology (2 mentions) Rhodamine 123 rho 123, by Merck Group (2 mentions) 1 4 5 dimethylthiazol 2 yl 3 5 diphenylformazan mtt, by Merck Group (2 mentions) Mitoxantrone, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)

7 protocols using sybr green qpcr master mix

Evaluating Drug Resistance Mechanisms

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CM082 was provided from Kanaji (Shanghai, China). Rho 123, MX, topotecan, DOX, DDP, FTC, Hoechst 33342, MTT, DMSO (dimethyl sulfoxide), and VRP (verapamil) were purchased from Sigma-Aldrich (St. Louis, MO, USA). FBS (fetal bovine serum), RPMI 1640, and DMEM (Dulbecco’s modified Eagle’s medium) were obtained from Gibco-BRL (Gaithersburg, MD, USA). Antibodies against phosphorylated (phospho-)AKT, phospho-ERK, ABCG2, total AKT, and total ERK were provided by Santa Cruz Biotechnology (Paso Robles, CA, USA). The antibody against GAPDH was provided by Kangcheng (Shanghai, People’s Republic of China). ExCell Bio (Shanghai, People’s Republic of China) provided SYBR Green qPCR master mix.

Xu L., Huang J., Liu J., Xi Y., Zheng Z., To K.K., Chen Z., Wang F., Zhang Y, & Fu L. (2019). CM082 Enhances the Efficacy of Chemotherapeutic Drugs by Inhibiting the Drug Efflux Function of ABCG2. Molecular Therapy Oncolytics, 16, 100-110.

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Investigating Alectinib Drug Resistance Mechanisms

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Alectinib was purchased from KareBay Biochem (Monmouth Junction, NJ, USA). The formula of Alectinib is presented in Figure 1a. Antibodies against ABCB1/p-gp (sc-13131), ABCG2 (sc-377176), AKT (sc-8312), p-AKT (sc-7985-R), ERK (sc-514302) and p-ERK (sc-7383) were from Santa Cruz Biotechnology Inc. (Paso Robles, CA, USA). Flow cytometry antibodies against ABCB1 (#557002) and mouse IgG2b/κ (#559532) were purchased from BD Biosciences (San Jose, CA, USA), and ABCG2 (sc-18841) was from Santa Cruz Biotechnology Inc. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was from Kangchen Co. (Shanghai, China). SYBR Green qPCR Master Mix was from ExCell Bio (Shanghai, China). Dulbecco's modified Eagle's medium and RPMI 1640 medium were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Rhodamine 123 (Rho 123), 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan (MTT), G418, paclitaxel, doxorubicin (DOX), vincristine (VCR), verapamil (VRP), topotecan, mitoxantrone (MX), cisplatin, fumitremorgin C (FTC) and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Yang K., Chen Y., To K.K., Wang F., Li D., Chen L, & Fu L. (2017). Alectinib (CH5424802) antagonizes ABCB1- and ABCG2-mediated multidrug resistance in vitro, in vivo and ex vivo. Experimental & Molecular Medicine, 49(3), e303-.

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Molecular Mechanisms of Drug Resistance

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The following drugs/chemicals, 3-(4,5-Dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT), Dox, paclitaxel, MX, topotecan, cisplatin, Rho 123, verapamil (VRP), fumitremorgin C (FTC), were purchased from Sigma-Aldrich (St. Louis, MO, USA). MRTX849 was obtained from MedChemExpress (New Jersey, NJ, USA). Antibodies against ABCB1, AKT, p-AKT, ERK, and p-ERK, GAPDH were obtained from Santa Cruz Biotechnology Inc. (Paso Robles, CA, USA). DMEM and RPMI 1640 medium were purchased from Gibco BRL (Gaithersburg, MD, USA). SYBR Green qPCR Master Mix was obtained from ExCell Bio (Shanghai, China).

Zhang Y., Li C., Xia C., Wah To K.K., Guo Z., Ren C., Wen L., Wang F., Fu L, & Liao N. (2022). Adagrasib, a KRAS G12C inhibitor, reverses the multidrug resistance mediated by ABCB1 in vitro and in vivo. Cell Communication and Signaling : CCS, 20, 142.

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Evaluating Anticancer Drug Efficacy

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The 3-(4,5-Dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), MX, topotecan, cisplatin (DDP), DOX, rhodamine123 (Rho 123), hoechst 33342, verapamil (VRP) and fumitremorgin C (FTC) were products of Sigma Aldrich Chemical Co (St. Louis, MO, USA). Dacomitinib (PF299804) and lapatinib were purchased from Selleck Chemicals (Houston, TX, USA). Dulbecco’s modified Eagle’s medium (DMEM), RPMI-1640 and fetal bovine serum (FBS) were products of Gibco BRL (Gaithersburg, MD, USA). Anti-total Akt, Anti-phospho-Akt, anti-total ERK1/2, anti-phospho-ERK1/2 antibodies and anti-ABCG2 antibodies were obtained from Santa Cruz Biotechnology (Paso Robles, CA, USA). The antibody against GAPDH was from Kangcheng (Shanghai, China). SYBR Green qPCR Master mix was purchased from ExCell Bio (Shanghai, China).

Guo X., To K.K., Chen Z., Wang X., Zhang J., Luo M., Wang F., Yan S, & Fu L. (2018). Dacomitinib potentiates the efficacy of conventional chemotherapeutic agents via inhibiting the drug efflux function of ABCG2 in vitro and in vivo. Journal of Experimental & Clinical Cancer Research : CR, 37, 31.

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Evaluating ABC Transporter Inhibitors

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ERK5-IN-1 was purchased from Selleck Chemicals (Houston, TX, USA). 1-(4, 5-dimethylthiazol-2-yl)-3, 5-diphenylformazan (MTT), vincristine, paclitaxel, verapamil, cisplatin, doxorubicin (Dox), mitoxantrone, fumitremorgin C (FTC) and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). DMEM and RPMI 1640 medium were obtained from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Antibodies against ABCB1/P-gp (sc-13,131), Akt (sc-8312), p-Akt (sc-7985-R), ERK 1/2 (sc-514,302), and p-ERK (sc-7383) were from Santa Cruz Biotechnology Inc. (Paso Robles, CA, USA). ABCG2 antibody (MAB4146) was obtained from Chemicon International, Inc. (Billerica, MA). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was from Kangcheng Co. (Shanghai, China). SYBR Green qPCR Master Mix was from ExCell Bio (Shanghai, China). Flow cytometry antibodies against ABCB1 (#557002) and Mouse IgG2b/κ (#559532) were purchased from BD Biosciences (San Jose, CA, USA).

Wang F., Li D., Zheng Z., Kin Wah To K., Chen Z., Zhong M., Su X., Chen L, & Fu L. (2020). Reversal of ABCB1-related multidrug resistance by ERK5-IN-1. Journal of Experimental & Clinical Cancer Research : CR, 39, 50.

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Evaluating Anti-Cancer Drug Resistance Mechanisms

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Dulbecco's modified Eagle medium (DMEM) and Gibco ® RPMI-1640 were purchased from Thermo Fisher Scientific Inc. (MA, USA). Chemicals 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), mitoxantrone, topotecan, doxorubicin, paclitaxel, verapamil, fumitremorgin C (FTC), and Rhodamine 123 were purchased from Sigma-Aldrich (MO, USA); PCI29732 was purchased from Med Chem Express (NJ, USA). Antibodies against ABCG2 (sc-377176), AKT (sc-8312), p-AKT (sc-7985-R), ERK (sc-514302), and p-ERK (sc-7383) were purchased from Santa Cruz Biotechnology, Inc. (CA, USA). Antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was purchased from Kangcheng Co. (Shanghai, China). SYBR Green qPCR Master Mix was purchased from ExCell Bio (Shanghai, China).

Ge C., Wang F., Cui C., Su X., To K.K.W., Wang X., Zhang H., Song X, & Fu L. (2018). PCI29732, a Bruton's Tyrosine Kinase Inhibitor, Enhanced the Efficacy of Conventional Chemotherapeutic Agents in ABCG2-Overexpressing Cancer Cells. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 48(6).

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Investigating Multidrug Resistance Mechanisms

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Lazertinib was purchased from MedChemExpress (Princeton, NJ, USA). Vincristine (VCR), paclitaxel, DOX, mitoxantrone (MX), topotecan, G418, fumitremorgin C (FTC), verapamil (VRP), cisplatin, rhodamine 123 (Rho 123), and 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan (MTT) were acquired from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against ABCB1, ABCG2, ERK, and p-ERK, Akt, p-Akt, and flow cytometry antibodies against ABCG2 were obtained from Santa Cruz Biotechnology Inc. (Paso Robles, CA, USA). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was obtained from Kangchen Co. (Shanghai, China). ABCB1 and mouse IgG2b/κ antibodies used in flow cytometry were obtained from BD Biosciences (San Jose, CA, USA). RPMI 1640 and DMEM media were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). SYBR Green qPCR Master Mix was obtained from ExCell Bio (Shanghai, China).

Fan Y., Tao T., Guo Z., Wah To K.K., Chen D., Wu S., Yang C., Li J., Luo M., Wang F, & Fu L. (2022). Lazertinib improves the efficacy of chemotherapeutic drugs in ABCB1 or ABCG2 overexpression cancer cells in vitro, in vivo, and ex vivo. Molecular Therapy Oncolytics, 24, 636-649.

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