Hemoglobin was measured by spectrophotometry with the sodium lauryl sulphate (SLS)-hemoglobin method using Sysmex XN 2000 (Sysmex Europe GmbH, Norderstedt, Germany). Platelets were measured by the direct current (DC) detection method with Sysmex XN 2000 (Sysmex Europe GmbH). Leucocytes were measured by flow cytometry Sysmex XN 2000 (Sysmex Europe GmbH). All reagents used in the Sysmex XN 2000 analyzer came from Sysmex Europe GmbH.
Xn 2000
Manufactured by Sysmex
Sourced in Japan, Germany, China
The XN-2000 is a hematology analyzer designed for high-volume clinical laboratories. It performs automated analysis of blood samples, providing accurate and reliable results for a variety of blood cell parameters.
Automatically generated - may contain errors
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91 protocols using xn 2000
1
Hematology Analyzer Measurements
2
Automated Hematological Analysis Protocol
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Hemoglobin was measured by spectrophotometry with the sodium lauryl sulphate (SLS)-hemoglobin method using Sysmex XN2000 (Sysmex Europe GmbH, Norderstedt, Germany). Erythrocytes and platelets were measured by the direct current (DC) detection method with Sysmex XN2000 (Sysmex Europe GmbH). Leucocytes, neutrocytes, lymphocytes, monocytes, eosinophils, and basophils were measured by flow cytometry Sysmex XN 2000 (Sysmex Europe GmbH). Mean corpusclar volume (MCV) was automatically calculated with the same device. All reagents used in the Sysmex XN 2000 analyzer came from Sysmex Europe GmbH.
3
Evaluating Immune Function Post-Intervention
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The following parameters were determined prior to the mechanical interventional procedure and 5±2 days after the procedure. White blood cell (WBC) count and a differential were performed on the Sysmex XN2000 analyzer with Sysmex diagnostics. Lymphocyte subpopulations in peripheral blood were analyzed using a Navios flow cytometer and Beckman-Coulter diagnostic antibody cocktail Tetrachrome 45/56/19/3 (Ref: 66070703), CD8 PC7 (Ref: 737661), CD4-Alexa Fluor 750 (Ref: A94682), CD16-RPE (Ref: A07766), CD3-FITC/anti-HLA-DR-PE (Ref: A07737), CD45-PC5 (Ref: A07785), and anti-HLA-DR-PC7 (Ref: B49180). Serum calprotectin concentrations were determined using a CalproLab ELISA kit (Calpro AS).
4
Hematological and Liver Function Biomarkers
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White blood cell (WBC), red blood cell (RBC) and platelet (PLT) counts were measured by XN2000 (SYSMEX, Kobe, Japan). Levels of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (TBIL) and albumin (ALB) were measured on a 7600‐020 (ISE) Automatic Analyzer (HITACHI, Tokyo, Japan). The APRI (AST, platelet ratio index) was calculated using routine laboratory values [APRI = (AST/upper limit of normal) × 100/platelet count],9 and FIB‐4 using the formula age (years) × AST (IU/L)/platelet count (109/L) × (ALT (IU/L)1/2.10
5
Comprehensive Blood Analysis in Sickle Cell Disease
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The selected SCD patients were screened for eligibility. Permission was obtained from caregivers whose child met the inclusion criteria, and caregivers or patients more than 7 years were consented or assented respectively. Five milliliters of blood samples were collected from each patient, i.e., 2 and 3 ml were transferred into an ethylenediaminetetraacetic acid (EDTA) vacutainer and serum separator (SS) tube, respectively. The samples were placed in a cool box containing ice packs and transported to the Central Research Laboratory, KNUST, within 6–8 h of collection for processing. The fresh EDTA blood samples were subjected to complete blood count analysis using a hematology autoanalyzer (XN‐2000; Sysmex). Erythrocyte sedimentation rate (ESR) was also performed using the fresh EDTA blood samples to screen for possible inflammation. The fresh EDTA blood samples Following centrifugation of the sample in the SS tube, the serum was separated and stored in a minus 80°C freezer until a pool sample of 178 was obtained for the ferritin assay. Serum ferritin was measured by Ferritin enzyme immunoassay test kit (Fortress Diagnostics) according to the manufacturer's instructions.
6
Comprehensive Hematological and Biochemical Profiling
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Baseline information, including age, sex, and medical and medication history, was collected from the medical records of all participants. A Sysmex XN2000 automatic hematology analyzer was used to measure white blood cells (WBC), neutrophils (NEU), monocytes (MON), lymphocytes (LYM), platelets (PLT), mean corpuscular volume (MCV), mean platelet volume (MPV), and red blood cell distribution width- coefficient of variation (RDW-CV). Albumin (ALB), prealbumin (pALB), alkaline phosphatase (ALP), total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), HDL-C, apolipoprotein A1 (APO-A1), and apolipoprotein B100 (APO-B100) levels were measured in fasting venous blood using a Hitachi 7600 automatic biochemical analyzer. C-reactive protein (CRP) levels were determined using an immunoturbidimetric assay with the IMMAGE 800 system.
7
Comprehensive Blood Analysis via Sysmex XN 2000
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Basic parameters of complete blood were analyzed using the Sysmex XN 2000 hematology system and dedicated diagnostic reagents. The population of white blood cells was determined by manual and automatic blood smears and presented in percentage of total cell count. The tests were carried out in the hospital laboratory on the day of the patient’s admission to the department.
8
Bacteremia Diagnostic Protocol Analysis
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Blood culture and WBC differential tests were performed at the discretion of the examining doctors and subsequently performed by an automated analyzer. The sample of the blood cell count were collected by drawing blood into a tube containing an ethylenediaminetetraacetic acid dipotassium. The measurements of the blood cell count, including band count, were analyzed using the Sysmex XN-2000 automatic blood cell analyzer.
We extracted data regarding age, sex, body temperature, WBC count, eosinophil count, percentage band count in the differential WBC test, and blood culture results from the electronic medical records. The primary outcome measurement was bacteremia, which was defined as a positive blood culture 1 week after the samples were collected [20 (link)]. We defined contamination as the presence of multiplying coagulase-negative Staphylococcus species, Bacillus species, Propionibacterium acnes, or Corynebacterium species in a single set of blood cultures [30 (link)]. Bandemia was defined as a band count of >10%, based on a previous study [8 (link)]. Eosinopenia, which has been reported as a risk of bacteremia, was set at a cutoff of 25 cells/mm3 [31 (link)].
We extracted data regarding age, sex, body temperature, WBC count, eosinophil count, percentage band count in the differential WBC test, and blood culture results from the electronic medical records. The primary outcome measurement was bacteremia, which was defined as a positive blood culture 1 week after the samples were collected [20 (link)]. We defined contamination as the presence of multiplying coagulase-negative Staphylococcus species, Bacillus species, Propionibacterium acnes, or Corynebacterium species in a single set of blood cultures [30 (link)]. Bandemia was defined as a band count of >10%, based on a previous study [8 (link)]. Eosinopenia, which has been reported as a risk of bacteremia, was set at a cutoff of 25 cells/mm3 [31 (link)].
9
Extracting Mouse Blood and Tissues
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The whole blood of mice was obtained by extracting the eyeball blood under anesthesia [39 (link)]. The whole blood of mice (containing 15% EDTA-2K) was collected for the measurement of blood routine indices (XN2000, SYSMEX, Kobe, Japan). On the other hand, after the whole blood was collected, the upper serum was collected by centrifugation for the measurement of biochemical indices (c702, Roche, Basel, Switzerland).
The peripheral blood of mice was obtained by extracting the eyeball blood. After the mice were sacrificed, liver, kidney and tumor tissues were obtained by dissection. The copper ion concentration determination experiment was completed by Shiyanjia Lab.
The peripheral blood of mice was obtained by extracting the eyeball blood. After the mice were sacrificed, liver, kidney and tumor tissues were obtained by dissection. The copper ion concentration determination experiment was completed by Shiyanjia Lab.
10
Platelet Aggregation Analysis Protocol
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Whole blood samples were taken from venipuncture into a vacutainer tube containing moderate EDTA at room temperature. Platelet counts were detected by automated hematology analyzer (XN2000, Sysmex, Japan) within 1 h after sample collection. Blood was collected into 3.2% sodium citrate (1:9), and PAgT was assessed by aggregation analyzer (AggRAM, Helena Laboratories, USA). To produce platelet-rich plasma (PRP), the sample tubes were centrifuged at 100 g for 15 min at room temperature (approximately 25 °C). The suitable PRP (450 μL) was removed to dedicated reaction cuvette and kept at room temperature until testing. Platelet-poor plasma (PPP) was made by further centrifugation of PRP, at 2400 g for 20 min. The PPP was aspirated as described for the PRP. The PRP and PPP were incubated for 5 min at 37 °C, respectively. Then PPP tube was inserted into the test hole and set the 100% aggregation rate. Magnetic beads were added into the PPP tube to calibrate the 0% aggregation rate. To measure the aggregation of PRP, 50 μL ADP (20 μM) was added, and the changes of light absorbance was monitored to record the aggregation process.
All methods in our study were carried out in accordance with relevant guidelines and regulations.
All methods in our study were carried out in accordance with relevant guidelines and regulations.
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